aspsirna0967 details

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ASPsiRNA information siRNA Id:aspsirna0967
siRNA Name: siHUNT1
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):CGGCGCUCAGCCGGGCUCCG
Mismatch position in siRNA: NA
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):GGTGCTGAGCGGCGCCGCGAGTCGGCCCGAGG
Wild allele (5'->3'):NA
Mutant allele (5'->3'):GCCGCGAGUCGGCCCGAGGC
Position of siRNA on target gene: 262-281
Respective Gene/Protein Resources GenBank Accession:L12392.1
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite/synonymous variant
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): GCCGCGAGUCGGCCCGAGGC
ASP-siRNA (As strand 3'->5'):CGGCGCUCAGCCGGGCUCCG
Percentage efficacy of ASP-siRNA for mutant allele:78
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CGGCGCUCAGCCGGGCUCCG
Percentage efficacy of ASP-siRNA for wild allele:52
Relative difference:26
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CGGCGCUCAGCCGGGCUCCG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Western blot
Transfection method: Lipofectamine
siRNA expression method: Invitrogen
Post-transfection duration: 48 hours
Concentration used:10mg
Reference:16019264
Delivery method:Transfection
Article title:Intrastriatal rAAV-mediated delivery of anti-huntingtin shRNAs induces partial reversal of disease progression in R6/1 Huntington's disease transgenic mice
Authors:Rodriguez-Lebron E1, Denovan-Wright EM, Nash K, Lewin AS, Mandel RJ
Journal Reference:Mol Ther. 2005 Oct;12(4):618-33