| | |
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| ASPsiRNA information |
siRNA Id: | aspsirna0967
|
|
siRNA Name: | siHUNT1
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | CGGCGCUCAGCCGGGCUCCG
|
|
Mismatch position in siRNA: | NA |
| Gene Information |
Gene Name | Hungtintin (HTT) |
|
Target Sequence (5'->3'): | GGTGCTGAGCGGCGCCGCGAGTCGGCCCGAGG
|
|
Wild allele (5'->3'): | NA
|
|
Mutant allele (5'->3'): | GCCGCGAGUCGGCCCGAGGC
|
|
Position of siRNA on target gene: | 262-281
|
| Respective Gene/Protein Resources |
GenBank Accession: | L12392.1 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | Q96CV9 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | rs19392295 |
| Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
|
Matation type/variant: | Microsatellite/synonymous variant
|
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Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
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Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
|
KEGG disease ID: | H00059 |
|
OMIM ID: | 143100 |
|
COSMIC: | HTT |
|
DECIPHER: | HTT |
|
GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | GCCGCGAGUCGGCCCGAGGC
|
|
ASP-siRNA (As strand 3'->5'): | CGGCGCUCAGCCGGGCUCCG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 78
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | CGGCGCUCAGCCGGGCUCCG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 52
|
|
Relative difference: | 26
|
| Wild siRNA details |
Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand 3'->5'): | NA
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | CGGCGCUCAGCCGGGCUCCG
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| Concentration used: | 10mg |
| Reference: | 16019264 |
| Delivery method: | Transfection |
'| Article title: | Intrastriatal rAAV-mediated delivery of anti-huntingtin shRNAs induces partial reversal of disease progression in R6/1 Huntington's disease transgenic mice |
'| Authors: | Rodriguez-Lebron E1, Denovan-Wright EM, Nash K, Lewin AS, Mandel RJ |
'| Journal Reference: | Mol Ther. 2005 Oct;12(4):618-33 |
'
