aspsirna0971 details

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ASPsiRNA information siRNA Id:aspsirna0971
siRNA Name: d7
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
Mismatch position in siRNA: NA
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
Wild allele (5'->3'):NA
Mutant allele (5'->3'):CAGCAGCAGCAGCAGCAGCAG
Position of siRNA on target gene: 367-387
Respective Gene/Protein Resources GenBank Accession:L12392.1
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite/synonymous variant
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): CAGCAGCAGCAGCAGCAGCAG
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
Percentage efficacy of ASP-siRNA for wild allele:60
Relative difference:15
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Metabion
Post-transfection duration: 24 hours
Concentration used:20nM
Reference:21427085
Delivery method:Transfection
Article title:Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats.
Authors:Fiszer A, Mykowska A, Krzyzosiak WJ.
Journal Reference:Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22.