aspsirna0974 details

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ASPsiRNA information siRNA Id:aspsirna0974
siRNA Name: d7
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAG
Mismatch position in siRNA: NA
Gene Information Gene Name Ribosomal protein L14 (RPL14)
Target Sequence (5'->3'):GATTTTGATCGTTTTAAAGTTATGAAGG
Wild allele (5'->3'):NA
Mutant allele (5'->3'):GUCGUCGUCGUCGUCGUCGUC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_003973.4
Cytogenic location:NA
Chromosomal coordinates:NA
UniProt ID:Q6IPH7
HUGO ID:10305
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: Pathogenic
Disease:Neurological disorder
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: RPL14
DECIPHER: RPL14
GeneTests: RPL14
ASP siRNA details Mutant allele (5'->3'): GUCGUCGUCGUCGUCGUCGUC
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of ASP-siRNA for mutant allele:68
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of ASP-siRNA for wild allele:15
Relative difference:53
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Metabion
Post-transfection duration: 24 hours
Concentration used:20nM
Reference:21427085
Delivery method:Transfection
Article title:Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats.
Authors:Fiszer A, Mykowska A, Krzyzosiak WJ.
Journal Reference:Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22.