aspsirna0981 details

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ASPsiRNA information siRNA Id:aspsirna0981
siRNA Name: d9
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAGCAGCAG
Mismatch position in siRNA: NA
Gene Information Gene Name Low density lipoprotein receptor-related protein 8 (LRP8)
Target Sequence (5'->3'):GACGGCGACCGCGACTGCAAAGACAAATCGGACGA
Wild allele (5'->3'):NA
Mutant allele (5'->3'):GUCGUCGUCGUCGUCGUCGUCGUCGUC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_004631.4
Cytogenic location:1p32.3
Chromosomal coordinates:1:53,708,035-53,793,820
UniProt ID:Q14114
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: Pathogenic
Disease:Neurological disorder
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: LRP8
DECIPHER: LRP8
GeneTests: LRP8
ASP siRNA details Mutant allele (5'->3'): GUCGUCGUCGUCGUCGUCGUCGUCGUC
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of ASP-siRNA for mutant allele:60
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of ASP-siRNA for wild allele:20
Relative difference:40
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CAGCAGCAGCAGCAGCAGCAGCAGCAG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Metabion
Post-transfection duration: 24 hours
Concentration used:20nM
Reference:21427085
Delivery method:Transfection
Article title:Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats.
Authors:Fiszer A, Mykowska A, Krzyzosiak WJ.
Journal Reference:Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22.