| | |
ASPsiRNA information |
siRNA Id: | aspsirna0983
|
siRNA Name: | W13
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUC
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCGUCGUC
|
Mismatch position in siRNA: | A9G |
Gene Information |
Gene Name | Hungtintin (HTT) |
Target Sequence (5'->3'): | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
|
Mutant allele (5'->3'): | CAGCAGCAGCAGUAGCAGCAG
|
Position of siRNA on target gene: | 367-387
|
Respective Gene/Protein Resources |
GenBank Accession: | L12392.1 |
Cytogenic location: | 4p16.3 |
Chromosomal coordinates: | 4:3,076,407-3,245,686 |
UniProt ID: | Q14114 |
HUGO ID: | 4851 |
Reference SNp(RefSNP): | rs19392295 |
Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
Matation type/variant: | Microsatellite
|
Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Huntington disease (HD) |
Clinical Resources |
ClinVar ID: | 31916 |
KEGG disease ID: | H00059 |
OMIM ID: | 143100 |
COSMIC: | HTT |
DECIPHER: | HTT |
GeneTests: | HTT |
ASP siRNA details |
Mutant allele (5'->3'): | CAGCAGCAGCAGUAGCAGCAG
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCGUCGUC
|
Percentage efficacy of ASP-siRNA for mutant allele: | 75
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCGUCGUC
|
Percentage efficacy of ASP-siRNA for wild allele: | 20
|
Relative difference: | 55
|
Wild siRNA details |
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
|
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUC
|
Percentage efficacy of wild siRNA for wild allele: | 75
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCGUCGUC
|
Percentage efficacy of Wild sirna for mutant allele: | 60
|
Relative difference : | 15
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | Fibroblast |
Experimental technique used: | RT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Metabion |
Post-transfection duration: | 24 hours |
Concentration used: | 20nM |
Reference: | 21427085 |
Delivery method: | Transfection |
'Article title: | Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats. |
'Authors: | Fiszer A, Mykowska A, Krzyzosiak WJ. |
'Journal Reference: | Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22. |
'