aspsirna0994 details

''''
ASPsiRNA information siRNA Id:aspsirna0994
siRNA Name: W13/16-asymm(17/21) -II
Mismatch information Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCAUCAUCGUC
Mismatch position in siRNA: A6G,A9G
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
Wild allele (5'->3'):CAGCAGCAGCAGCAGCA
Mutant allele (5'->3'):CAGCAGCAGCAGUAGUA
Position of siRNA on target gene: 367-383
Respective Gene/Protein Resources GenBank Accession:L12392.1
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:P54252
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): CAGCAGCAGCAGUAGUA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCAUCAUCGUC
Percentage efficacy of ASP-siRNA for mutant allele:78
Wild allele (5'->3'): CAGCAGCAGCAGCAGCA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCAUCAUCGUC
Percentage efficacy of ASP-siRNA for wild allele:25
Relative difference:53
Wild siRNA details Wild allele (5'->3'): CAGCAGCAGCAGCAGCA
Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUC
Percentage efficacy of wild siRNA for wild allele:75
Wild allele (5'->3'): CAGCAGCAGCAGCAGCA
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGUCAUCAUCGUC
Percentage efficacy of Wild sirna for mutant allele:55
Relative difference :20
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Metabion
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:21427085
Delivery method:Transfection
Article title:Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats.
Authors:Fiszer A, Mykowska A, Krzyzosiak WJ.
Journal Reference:Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22.