| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna0994
|
|
siRNA Name: | W13/16-asymm(17/21) -II
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUC
|
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCAUCGUC
|
|
Mismatch position in siRNA: | A6G,A9G |
| Gene Information |
Gene Name | Hungtintin (HTT) |
|
Target Sequence (5'->3'): | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCA
|
|
Mutant allele (5'->3'): | CAGCAGCAGCAGUAGUA
|
|
Position of siRNA on target gene: | 367-383
|
| Respective Gene/Protein Resources |
GenBank Accession: | L12392.1 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | P54252 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | rs19392295 |
| Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
|
Matation type/variant: | Microsatellite
|
|
Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
|
Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
|
KEGG disease ID: | H00059 |
|
OMIM ID: | 143100 |
|
COSMIC: | HTT |
|
DECIPHER: | HTT |
|
GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | CAGCAGCAGCAGUAGUA
|
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCAUCGUC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 78
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCA
|
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCAUCGUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 25
|
|
Relative difference: | 53
|
| Wild siRNA details |
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCA
|
|
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUC
|
|
Percentage efficacy of wild siRNA for wild allele: | 75
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCA
|
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCAUCAUCGUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 55
|
|
Relative difference : | 20
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Metabion |
| Post-transfection duration: | 24 hours |
| Concentration used: | 100nM |
| Reference: | 21427085 |
| Delivery method: | Transfection |
'| Article title: | Inhibition of mutant huntingtin expression by RNA duplex targeting expanded CAG repeats. |
'| Authors: | Fiszer A, Mykowska A, Krzyzosiak WJ. |
'| Journal Reference: | Nucleic Acids Res. 2011 Jul;39(13):5578-85. doi: 10.1093/nar/gkr156. Epub 2011 Mar 22. |
'
