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| ASPsiRNA information |
siRNA Id: | aspsirna0999
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siRNA Name: | HAT DsRNA
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CACGUCGCCGCCGCGCCGCGC
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ASP-siRNA (As strand 3'->5'): | CACGCCGCCGCCGCGCCGCGC
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Mismatch position in siRNA: | C17U |
| Gene Information |
Gene Name | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1(CITED1) |
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Target Sequence (5'->3'): | GGCACTGTGCGGUGCAGCGGCGGCGCGGCGCGGTGCGGG
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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Mutant allele (5'->3'): | GUGCGGCGGCGGCGCGGCGCG
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Position of siRNA on target gene: | 306-326
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_004143.3 |
| Cytogenic location: | Xq13.1 |
| Chromosomal coordinates: | X:71,521,487-71,527,036 |
| UniProt ID: | P34545 |
| HUGO ID: | 1546 |
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Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | c.211A>G
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Matation type/variant: | NA
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Mutation at gene level: | NA
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Pathogenic status of mutation: | Pathogenic
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | NA |
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KEGG disease ID: | H00001 |
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OMIM ID: | 600140 |
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COSMIC: | CITED1 |
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DECIPHER: | CITED1 |
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GeneTests: | CITED1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GUGCGGCGGCGGCGCGGCGCG
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ASP-siRNA (As strand 3'->5'): | CACGCCGCCGCCGCGCCGCGC
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Percentage efficacy of ASP-siRNA for mutant allele: | 68
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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ASP-siRNA (As strand 3'->5'): | CACGCCGCCGCCGCGCCGCGC
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Percentage efficacy of ASP-siRNA for wild allele: | 38
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Relative difference: | 30
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| Wild siRNA details |
Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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Wild siRNA (As strand 3'->5'): | CACGUCGCCGCCGCGCCGCGC
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Percentage efficacy of wild siRNA for wild allele: | Lowest effective
|
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
|
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ASP-siRNA (As strand 3'->5'): | CACGCCGCCGCCGCGCCGCGC
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Percentage efficacy of Wild sirna for mutant allele: | 68
|
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Relative difference : | Poor discrimination
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| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | N2 wild-type worms (C.Elegans) |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Promega |
| Post-transfection duration: | 24 hours |
| Concentration used: | 10mM |
| Reference: | 11751575 |
| Delivery method: | Transfection |
'| Article title: | HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans. |
'| Authors: | Victor M, Bei Y, Gay F, Calvo D, Mello C, Shi Y. |
'| Journal Reference: | EMBO Rep. 2002 Jan;3(1):50-5. Epub 2001 Dec 19. |
'
