aspsirna1001 details

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ASPsiRNA information siRNA Id:aspsirna1001
siRNA Name: P10mut
Mismatch information Wild siRNA (As strand 3'->5'):CCUCUGAACCCGUUACACU
ASP-siRNA (As strand 3'->5'):CCUCUGAACGCGUUACACU
Mismatch position in siRNA: G10C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):GAGAGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAG
Wild allele (5'->3'):GGAGACUUGGGCAAUGUGA
Mutant allele (5'->3'):GGAGACUUGCGCAAUGUGA
Position of siRNA on target gene: 395-413
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.11
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.256G>C,p.Gly86Arg
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12653G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14758
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): GGAGACUUGCGCAAUGUGA
ASP-siRNA (As strand 3'->5'):CCUCUGAACGCGUUACACU
Percentage efficacy of ASP-siRNA for mutant allele:80
Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
ASP-siRNA (As strand 3'->5'):CCUCUGAACGCGUUACACU
Percentage efficacy of ASP-siRNA for wild allele:20
Relative difference:60
Wild siRNA details Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
Wild siRNA (As strand 3'->5'):CCUCUGAACCCGUUACACU
Percentage efficacy of wild siRNA for wild allele:Lowest effective
Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
ASP-siRNA (As strand 3'->5'):CCUCUGAACGCGUUACACU
Percentage efficacy of Wild sirna for mutant allele:93
Relative difference :High discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Transgenic mouse model
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:12934714
Delivery method:Hydrodynamic transfection
Article title:Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis.
Authors:Ding H, Schwarz DS, Keene A, Affar el B, Fenton L, Xia X, Shi Y, Zamore PD, Xu Z.
Journal Reference:Aging Cell. 2003 Aug;2(4):209-17.