aspsirna1012 details

''''
ASPsiRNA information siRNA Id:aspsirna1012
siRNA Name: siRNAT1
Mismatch information Wild siRNA (As strand 3'->5'):GACUUGGGGGGGUUUUCGG
ASP-siRNA (As strand 3'->5'):GACUUGGGGGAGUUUUCGG
Mismatch position in siRNA: A11G
Gene Information Gene Name Collagen type I alpha 1 (COL1A1)
Target Sequence (5'->3'):AACTGAACCCCCTCAAAAGCC
Wild allele (5'->3'):CUGAACCCCCCCAAAAGCC
Mutant allele (5'->3'):CUGAACCCCCUCAAAAGCC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_000088.3
Cytogenic location:17q21.33
Chromosomal coordinates:17:48,261,456-48,279,002
UniProt ID:P02452
HUGO ID:2197
Reference SNp(RefSNP): rs1061237
Disease/Mutation information Target Mutation: c.*88T>C
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_007400.1:g.21226T>C
Pathogenic status of mutation: Pathogenic
Disease:Osteogenesis Imperfecta (OI)
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00506
OMIM ID: 166210
COSMIC: COL1A1
DECIPHER: COL1A1
GeneTests: COL1A1
ASP siRNA details Mutant allele (5'->3'): CUGAACCCCCUCAAAAGCC
ASP-siRNA (As strand 3'->5'):GACUUGGGGGAGUUUUCGG
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): CUGAACCCCCCCAAAAGCC
ASP-siRNA (As strand 3'->5'):GACUUGGGGGAGUUUUCGG
Percentage efficacy of ASP-siRNA for wild allele:64
Relative difference:11
Wild siRNA details Wild allele (5'->3'): CUGAACCCCCCCAAAAGCC
Wild siRNA (As strand 3'->5'):GACUUGGGGGGGUUUUCGG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): CUGAACCCCCCCAAAAGCC
ASP-siRNA (As strand 3'->5'):GACUUGGGGGAGUUUUCGG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Cos-7
Experimental technique used: ELISA assay
Transfection method: Lipofectamine 2000
siRNA expression method: Qiagen
Post-transfection duration: 24 hours
Concentration used:50nMol
Reference:15241481
Delivery method:Transfection
Article title:RNAi of COL1A1 in mesenchymal progenitor cells.
Authors:Millington-Ward S, McMahon HP, Allen D, Tuohy G, Kiang AS, Palfi A, Kenna PF, Humphries P, Farrar GJ.
Journal Reference:Eur J Hum Genet. 2004 Oct;12(10):864-6