aspsirna1031 details

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ASPsiRNA information siRNA Id:aspsirna1031
siRNA Name: R1+R4
Mismatch information Wild siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUG?
ASP-siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUU
Mismatch position in siRNA: U1G(S),A16U(S)
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):AGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAA
Wild allele (5'->3'):GAGACUUGGGCAAUGUGAC
Mutant allele (5'->3'):GAGACUUGGGCAAUGAGAC
Position of siRNA on target gene: 396-414
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.11
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.256G>C,p.Gly86Arg
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12653G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14758
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): GAGACUUGGGCAAUGAGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUU
Percentage efficacy of ASP-siRNA for mutant allele:45
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUU
Percentage efficacy of ASP-siRNA for wild allele:45
Relative difference:0
Wild siRNA details Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
Wild siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUG?
Percentage efficacy of wild siRNA for wild allele:8
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUU
Percentage efficacy of Wild sirna for mutant allele:80
Relative difference :72
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:2.0microg/mL
Reference:18215350
Delivery method:Electroporation
Article title:Double-mismatched siRNAs enhance selective gene silencing of a mutant ALS-causing allele.
Authors:Geng CM, Ding HL.
Journal Reference:Acta Pharmacol Sin. 2008 Feb;29(2):211-6. doi: 10.1111/j.1745-7254.2008.00740.x.