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| ASPsiRNA information |
siRNA Id: | aspsirna1035
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|
siRNA Name: | L4+R4
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUCUGAACCCGUUACACUG?
|
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ASP-siRNA (As strand 3'->5'): | CUCAGAACCCGUUACACUG
|
|
Mismatch position in siRNA: | A16U,A16U(S) |
| Gene Information |
Gene Name | Cu,Zn superoxide dismutase (SOD1) |
|
Target Sequence (5'->3'): | AGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAA
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Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
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Mutant allele (5'->3'): | GAGACUUGGGCAAUGAGAC
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|
Position of siRNA on target gene: | 396-414
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000454.4 |
| Cytogenic location: | 21q22.11 |
| Chromosomal coordinates: | 21:33,031,934-33,041,243 |
| UniProt ID: | P00441 |
| HUGO ID: | 11179 |
|
Reference SNp(RefSNP): | rs12191243 |
| Disease/Mutation information |
Target Mutation: | c.256G>C,p.Gly86Arg
|
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_008689.1:g.12653G>C
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Pathogenic status of mutation: | Pathogenic
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Disease: | Amyotrophic Lateral Sclerosis (ALS) |
| Clinical Resources |
ClinVar ID: | 14758 |
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KEGG disease ID: | H00058 |
|
OMIM ID: | 105400 |
|
COSMIC: | SOD1 |
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DECIPHER: | SOD1 |
|
GeneTests: | SOD1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GAGACUUGGGCAAUGAGAC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGAACCCGUUACACUG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 76
|
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Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGAACCCGUUACACUG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 58
|
|
Relative difference: | 18
|
| Wild siRNA details |
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
Wild siRNA (As strand 3'->5'): | CUCUGAACCCGUUACACUG?
|
|
Percentage efficacy of wild siRNA for wild allele: | 8
|
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
ASP-siRNA (As strand 3'->5'): | CUCAGAACCCGUUACACUG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 80
|
|
Relative difference : | -72
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| Concentration used: | 2.0microg/mL |
| Reference: | 18215350 |
| Delivery method: | Electroporation |
'| Article title: | Double-mismatched siRNAs enhance selective gene silencing of a mutant ALS-causing allele. |
'| Authors: | Geng CM, Ding HL. |
'| Journal Reference: | Acta Pharmacol Sin. 2008 Feb;29(2):211-6. doi: 10.1111/j.1745-7254.2008.00740.x. |
'
