aspsirna1545 details

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ASPsiRNA information siRNA Id:aspsirna1545
siRNA Name: siCD46-4C6A
Mismatch information Wild siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUAGCGCU
Mismatch position in siRNA: 4C,6A
Gene Information Gene Name Complement regulatory protein (CD46)
Target Sequence (5'->3'):CTTATTGGAGAGAGCACGATTTATTGTGGTGACA
Wild allele (5'->3'):CUUAUUGGAGAGAGCACGA
Mutant allele (5'->3'):CUUAUUGGAGAGAUCGCGA
Position of siRNA on target gene: 779-797
Respective Gene/Protein Resources GenBank Accession:NM_002389.4
Cytogenic location:1q32.2
Chromosomal coordinates:1:207,925,382-207,968,860
UniProt ID:P15529
HUGO ID:6953
Reference SNp(RefSNP): rs12190959
Disease/Mutation information Target Mutation: NM_002389.4:c.104G>A, p.Cys35Tyr
Matation type/variant: single nucleotide variant/Missense variant
Mutation at gene level: NG_009296.1:g.9964G>A
Pathogenic status of mutation: Risk factor
Disease:Hemolytic uremic syndrome
Clinical Resources ClinVar ID:17049
KEGG disease ID:H00106, H0027
OMIM ID: 120920
COSMIC: CD46
DECIPHER: CD46
GeneTests: CD46
ASP siRNA details Mutant allele (5'->3'): CUUAUUGGAGAGAUCGCGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUAGCGCU
Percentage efficacy of ASP-siRNA for mutant allele:59
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUAGCGCU
Percentage efficacy of ASP-siRNA for wild allele:38
Relative difference:21
Wild siRNA details Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
Wild siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUAGCGCU
Percentage efficacy of Wild sirna for mutant allele:38
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:Target gene
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:18420656
Delivery method:Transfection
Article title:Analysis of siRNA specificity on targets with double-nucleotide mismatches.
Authors:Dahlgren C, Zhang HY, Du Q, Grahn M, Norstedt G, Wahlestedt C, Liang Z.
Journal Reference:Nucleic Acids Res. 2008 May;36(9):e53. doi: 10.1093/nar/gkn190. Epub 2008 Apr 17.