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| ASPsiRNA information |
siRNA Id: | aspsirna1548
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siRNA Name: | siCD46-14T17A
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA (As strand 3'->5'): | GAAUAUCCUCUCUCGUGCU
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|
Mismatch position in siRNA: | 14U,17A |
| Gene Information |
Gene Name | Complement regulatory protein (CD46) |
|
Target Sequence (5'->3'): | CTTATTGGAGAGAGCACGATTTATTGTGGTGACA
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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Mutant allele (5'->3'): | CUUAUAGGAGAGAGCACGA
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Position of siRNA on target gene: | 779-797
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002389.4 |
| Cytogenic location: | 1q32.2 |
| Chromosomal coordinates: | 1:207,925,382-207,968,860 |
| UniProt ID: | P15529 |
| HUGO ID: | 6953 |
|
Reference SNp(RefSNP): | rs12190959 |
| Disease/Mutation information |
Target Mutation: | NM_002389.4:c.104G>A, p.Cys35Tyr
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Matation type/variant: | single nucleotide variant/Missense variant
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Mutation at gene level: | NG_009296.1:g.9964G>A
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Pathogenic status of mutation: | Risk factor
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Disease: | Hemolytic uremic syndrome |
| Clinical Resources |
ClinVar ID: | 17049 |
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KEGG disease ID: | H00106, H0027 |
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OMIM ID: | 120920 |
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COSMIC: | CD46 |
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DECIPHER: | CD46 |
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GeneTests: | CD46 |
| ASP siRNA details |
Mutant allele (5'->3'): | CUUAUAGGAGAGAGCACGA
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ASP-siRNA (As strand 3'->5'): | GAAUAUCCUCUCUCGUGCU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 24
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand 3'->5'): | GAAUAUCCUCUCUCGUGCU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 42
|
|
Relative difference: | -18
|
| Wild siRNA details |
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
Wild siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUAUCCUCUCUCGUGCU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 38
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | Target gene |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| Concentration used: | 13nM |
| Reference: | 18420656 |
| Delivery method: | Transfection |
'| Article title: | Analysis of siRNA specificity on targets with double-nucleotide mismatches. |
'| Authors: | Dahlgren C, Zhang HY, Du Q, Grahn M, Norstedt G, Wahlestedt C, Liang Z. |
'| Journal Reference: | Nucleic Acids Res. 2008 May;36(9):e53. doi: 10.1093/nar/gkn190. Epub 2008 Apr 17. |
'
