aspsirna1747 details

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ASPsiRNA information siRNA Id:aspsirna1747
siRNA Name: BetaA(G:G)-siRNA
Mismatch information Wild siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
ASP-siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
Mismatch position in siRNA: A1C
Gene Information Gene Name Hb beta-chain (HBB)
Target Sequence (5'->3'):ATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCC
Wild allele (5'->3'):UGACUCCUGAGGAGAAGUC
Mutant allele (5'->3'):UGACUCCUGAGGAGAAGUG
Position of siRNA on target gene: 61-79
Respective Gene/Protein Resources GenBank Accession:NM_000518.4
Cytogenic location:11p15.4
Chromosomal coordinates:11:5,246,695-5,248,300
UniProt ID:P68871
HUGO ID:4827
Reference SNp(RefSNP): rs33987957
Disease/Mutation information Target Mutation: c.365A>T (p.Glu122Val)
Matation type/variant: single nucleotide variant/Missense variant
Mutation at gene level: NG_000007.3:g.71939A>T
Pathogenic status of mutation: Pathogenic
Disease:Sickle cell anemia
Clinical Resources ClinVar ID:15110
KEGG disease ID:H00229
OMIM ID: 603903
COSMIC: HBB
DECIPHER: HBB
GeneTests: HBB
ASP siRNA details Mutant allele (5'->3'): UGACUCCUGAGGAGAAGUG
ASP-siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): UGACUCCUGAGGAGAAGUC
ASP-siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
Percentage efficacy of ASP-siRNA for wild allele:12
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): UGACUCCUGAGGAGAAGUC
Wild siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UGACUCCUGAGGAGAAGUC
ASP-siRNA (As strand 3'->5'):ACUGAGGACUCCUCUUCAG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:200nM
Reference:16585504
Delivery method:Transfection
Article title:Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism.
Authors:Dykxhoorn DM, Schlehuber LD, London IM, Lieberman J.
Journal Reference:Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5953-8. Epub 2006 Apr 3.