aspsirna1767 details

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ASPsiRNA information siRNA Id:aspsirna1767
siRNA Name: La21-5'm2
Mismatch information Wild siRNA (As strand 3'->5'):UGGCGACCUCUCGUUGACG
ASP-siRNA (As strand 3'->5'):AUGCGACCUCUCGUUGACG
Mismatch position in siRNA: A19U,U18C
Gene Information Gene Name P. luciferase
Target Sequence (5'->3'):GCUCAACGAGAGGUCGCCA
Wild allele (5'->3'):ACCGCUGGAGAGCAACUGC
Mutant allele (5'->3'):UACGCUGGAGAGCAAUAUU
Position of siRNA on target gene: 364-382
Respective Gene/Protein Resources GenBank Accession:M15077.1
Cytogenic location:NA
Chromosomal coordinates:NA
UniProt ID:P08659
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: P. luciferase
DECIPHER: P. luciferase
GeneTests: P. luciferase
ASP siRNA details Mutant allele (5'->3'): UACGCUGGAGAGCAAUAUU
ASP-siRNA (As strand 3'->5'):AUGCGACCUCUCGUUGACG
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): ACCGCUGGAGAGCAACUGC
ASP-siRNA (As strand 3'->5'):AUGCGACCUCUCGUUGACG
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): ACCGCUGGAGAGCAACUGC
Wild siRNA (As strand 3'->5'):UGGCGACCUCUCGUUGACG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): ACCGCUGGAGAGCAACUGC
ASP-siRNA (As strand 3'->5'):AUGCGACCUCUCGUUGACG
Percentage efficacy of Wild sirna for mutant allele:98
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Proligo
Post-transfection duration: 48 hours
Concentration used:40nM
Reference:14741366
Delivery method:Transfection
Article title:Enhancement of RNAi activity by improved siRNA duplexes.
Authors:Hohjoh H.
Journal Reference:FEBS Lett. 2004 Jan 16;557(1-3):193-8.