aspsirna1770 details

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ASPsiRNA information siRNA Id:aspsirna1770
siRNA Name: Lam-3'm2m12
Mismatch information Wild siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
ASP-siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
Mismatch position in siRNA: A1C,A2U,A8C
Gene Information Gene Name Human lamin A/C (LMNA)
Target Sequence (5'->3'):UGCUGAGAGGAACAGCAAC
Wild allele (5'->3'):UGCUGAGAGGAACAGCAACCU
Mutant allele (5'->3'):UGCUGAGUGGAACAGCAUU
Position of siRNA on target gene: 1080-1100
Respective Gene/Protein Resources GenBank Accession:NM_170707.3
Cytogenic location:1q22
Chromosomal coordinates:1:156,052,368-156,109,879
UniProt ID:P02545
HUGO ID:6636
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: LMNA
DECIPHER: LMNA
GeneTests: LMNA
ASP siRNA details Mutant allele (5'->3'): UGCUGAGUGGAACAGCAUU
ASP-siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
ASP-siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
Wild siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
ASP-siRNA (As strand 3'->5'):AGACGACUCUCCUUGUCGUUG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :70
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: JetSI transfection reagent
siRNA expression method: Proligo
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:14741366
Delivery method:Transfection
Article title:Enhancement of RNAi activity by improved siRNA duplexes.
Authors:Hohjoh H.
Journal Reference:FEBS Lett. 2004 Jan 16;557(1-3):193-8.