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| ASPsiRNA information |
siRNA Id: | aspsirna1776
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siRNA Name: | Dn1(#2)-3'm2m12
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
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ASP-siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
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Mismatch position in siRNA: | U1A,A8C,U2A |
| Gene Information |
Gene Name | DNA (cytosine-5-)-methyltransferase 1 (DNMT1) |
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Target Sequence (5'->3'): | AAAGAATCAGTTATGTGACTTGGAAACCAAATT
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Wild allele (5'->3'): | GUGACUUGGAAACCAAAUU
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Mutant allele (5'->3'): | GUGACUUUGAAACCAAAAA
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Position of siRNA on target gene: | 365-383
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_001379 |
| Cytogenic location: | 19p13.2 |
| Chromosomal coordinates: | 19:10,244,020-10,305,782 |
| UniProt ID: | P26358 |
| HUGO ID: | 2976 |
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Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
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Matation type/variant: | NA
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Mutation at gene level: | NA
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Pathogenic status of mutation: | NA
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Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
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KEGG disease ID: | NA |
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OMIM ID: | NA |
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COSMIC: | DNMT1 |
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DECIPHER: | DNMT1 |
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GeneTests: | DNMT1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GUGACUUUGAAACCAAAAA
|
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ASP-siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
|
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Percentage efficacy of ASP-siRNA for mutant allele: | NA
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Wild allele (5'->3'): | GUGACUUGGAAACCAAAUU
|
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ASP-siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
|
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Percentage efficacy of ASP-siRNA for wild allele: | NA
|
|
Relative difference: | NA
|
| Wild siRNA details |
Wild allele (5'->3'): | GUGACUUGGAAACCAAAUU
|
|
Wild siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | GUGACUUGGAAACCAAAUU
|
|
ASP-siRNA (As strand 3'->5'): | CACUGAACCUUUGGUUUAA
|
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Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | 70
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | JetSI transfection reagent |
| siRNA expression method: | Proligo |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100nM |
| Reference: | 14741366 |
| Delivery method: | Transfection |
'| Article title: | Enhancement of RNAi activity by improved siRNA duplexes. |
'| Authors: | Hohjoh H. |
'| Journal Reference: | FEBS Lett. 2004 Jan 16;557(1-3):193-8. |
'
