aspsirna1776 details

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ASPsiRNA information siRNA Id:aspsirna1776
siRNA Name: Dn1(#2)-3'm2m12
Mismatch information Wild siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
ASP-siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
Mismatch position in siRNA: U1A,A8C,U2A
Gene Information Gene Name DNA (cytosine-5-)-methyltransferase 1 (DNMT1)
Target Sequence (5'->3'):AAAGAATCAGTTATGTGACTTGGAAACCAAATT
Wild allele (5'->3'):GUGACUUGGAAACCAAAUU
Mutant allele (5'->3'):GUGACUUUGAAACCAAAAA
Position of siRNA on target gene: 365-383
Respective Gene/Protein Resources GenBank Accession:NM_001379
Cytogenic location:19p13.2
Chromosomal coordinates:19:10,244,020-10,305,782
UniProt ID:P26358
HUGO ID:2976
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: DNMT1
DECIPHER: DNMT1
GeneTests: DNMT1
ASP siRNA details Mutant allele (5'->3'): GUGACUUUGAAACCAAAAA
ASP-siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): GUGACUUGGAAACCAAAUU
ASP-siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): GUGACUUGGAAACCAAAUU
Wild siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): GUGACUUGGAAACCAAAUU
ASP-siRNA (As strand 3'->5'):CACUGAACCUUUGGUUUAA
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :70
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: JetSI transfection reagent
siRNA expression method: Proligo
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:14741366
Delivery method:Transfection
Article title:Enhancement of RNAi activity by improved siRNA duplexes.
Authors:Hohjoh H.
Journal Reference:FEBS Lett. 2004 Jan 16;557(1-3):193-8.