aspsirna1784 details

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ASPsiRNA information siRNA Id:aspsirna1784
siRNA Name: si10T
Mismatch information Wild siRNA (As strand 3'->5'):CGACGCGAUGCCGCUUCUG
ASP-siRNA (As strand 3'->5'):CGACGCGAUACCGCUUCUG
Mismatch position in siRNA: A10G
Gene Information Gene Name Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A)
Target Sequence (5'->3'):TTCCACGCCATGGGGGGTCGTGAGGGGCTCATTGACA
Wild allele (5'->3'):GCUGCGCUACGGCGAAGAC
Mutant allele (5'->3'):GCUGCGCUAUGGCGAAGAC
Position of siRNA on target gene: 2898-2934
Respective Gene/Protein Resources GenBank Accession:NM_000937.4
Cytogenic location:17p13.1
Chromosomal coordinates:17:7,387,697-7,417,934
UniProt ID:P24928
HUGO ID:9187
Reference SNp(RefSNP): rs14801110
Disease/Mutation information Target Mutation: c.2907C>T,(p.Ile969=)
Matation type/variant: single nucleotide variant/synonymous variant
Mutation at gene level: NG_027747.1:g.23893C>T
Pathogenic status of mutation: Not provided
Disease:Cancer
Clinical Resources ClinVar ID:80702
KEGG disease ID:H00014
OMIM ID: 180660
COSMIC: POLR2A
DECIPHER: POLR2A
GeneTests: POLR2A
ASP siRNA details Mutant allele (5'->3'): GCUGCGCUAUGGCGAAGAC
ASP-siRNA (As strand 3'->5'):CGACGCGAUACCGCUUCUG
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): GCUGCGCUACGGCGAAGAC
ASP-siRNA (As strand 3'->5'):CGACGCGAUACCGCUUCUG
Percentage efficacy of ASP-siRNA for wild allele:60
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): GCUGCGCUACGGCGAAGAC
Wild siRNA (As strand 3'->5'):CGACGCGAUGCCGCUUCUG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): GCUGCGCUACGGCGAAGAC
ASP-siRNA (As strand 3'->5'):CGACGCGAUACCGCUUCUG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: 15PC3
Experimental technique used: Northern blot
Transfection method: Lipofectamine 2000
siRNA expression method: Proligo
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:19165236
Delivery method:Transfection
Article title:Allele-specific cancer cell killing in vitro and in vivo targeting a single-nucleotide polymorphism in POLR2A.
Authors:Mook OR, Baas F, de Wissel MB, Fluiter K.
Journal Reference:Cancer Gene Ther. 2009 Jun;16(6):532-8. doi: 10.1038/cgt.2008.104. Epub 2009 Jan 23.
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ASPsiRNA information siRNA Id:aspsirna1784
siRNA Name: siCD46
Mismatch information Wild siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Mismatch position in siRNA: A:C clash
Gene Information Gene Name Complement regulatory protein (CD46)
Target Sequence (5'->3'):GCCCCCAATATGTGAAAAG
Wild allele (5'->3'):CUUAUUGGAGAGAGCACGA
Mutant allele (5'->3'):CCUAUUGGAGAGAGCACGA
Position of siRNA on target gene: 779-797
Respective Gene/Protein Resources GenBank Accession:NM_002389.4
Cytogenic location:1q32.2
Chromosomal coordinates:1:207,925,382-207,968,860
UniProt ID:P15529
HUGO ID:6953
Reference SNp(RefSNP): rs12190959
Disease/Mutation information Target Mutation: c.104G>A, p.Cys35Tyr
Matation type/variant: single nucleotide variant/Missense variant
Mutation at gene level: NG_009296.1:g.9964G>A
Pathogenic status of mutation: Risk factor
Disease:Hemolytic uremic syndrome
Clinical Resources ClinVar ID:17049
KEGG disease ID:H00106, H0027
OMIM ID: 120920
COSMIC: CD46
DECIPHER: CD46
GeneTests: CD46
ASP siRNA details Mutant allele (5'->3'): CCUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of ASP-siRNA for mutant allele:83
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of ASP-siRNA for wild allele:93
Relative difference:10
Wild siRNA details Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
Wild siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of wild siRNA for wild allele:93
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of Wild sirna for mutant allele:83
Relative difference :10
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:15781493
Delivery method:Transfection
Article title:A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites.
Authors:Du Q, Thonberg H, Wang J, Wahlestedt C, Liang Z.
Journal Reference:Nucleic Acids Res. 2005 Mar 21;33(5):1671-7. Print 2005. Erratum in: Nucleic Acids Res. 2005;33(11):3698.