aspsirna1825 details

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ASPsiRNA information siRNA Id:aspsirna1825
siRNA Name: W3/7 Fe775i
Mismatch information Wild siRNA (As strand 3'->5'):UUCGUCGUGUUACUACUCACG
ASP-siRNA (As strand 3'->5'):UUCGUCGUGUUACUGCUCGCG
Mismatch position in siRNA: G3A,G7A
Gene Information Gene Name Flap structure-specific endonuclease 1 (Fen1)
Target Sequence (5'->3'):GCAGCACAAUGACGAGCGCAA
Wild allele (5'->3'):AAGCAGCACAAUGAUGAGUGC
Mutant allele (5'->3'):AAGCAGCACAAUGACGAGCGC
Position of siRNA on target gene: 816-836
Respective Gene/Protein Resources GenBank Accession:NM_004111.5
Cytogenic location:11q12.2
Chromosomal coordinates:11:61,560,108-61,564,715
UniProt ID:P39748
HUGO ID:3650
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: Mutation tolerance study
Disease:Werner syndrome
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00296
OMIM ID: 277700
COSMIC: Fen1
DECIPHER: Fen1
GeneTests: Fen1
ASP siRNA details Mutant allele (5'->3'): AAGCAGCACAAUGACGAGCGC
ASP-siRNA (As strand 3'->5'):UUCGUCGUGUUACUGCUCGCG
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): AAGCAGCACAAUGAUGAGUGC
ASP-siRNA (As strand 3'->5'):UUCGUCGUGUUACUGCUCGCG
Percentage efficacy of ASP-siRNA for wild allele:22
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): AAGCAGCACAAUGAUGAGUGC
Wild siRNA (As strand 3'->5'):UUCGUCGUGUUACUACUCACG
Percentage efficacy of wild siRNA for wild allele:Moderately effective
Wild allele (5'->3'): AAGCAGCACAAUGAUGAGUGC
ASP-siRNA (As strand 3'->5'):UUCGUCGUGUUACUGCUCGCG
Percentage efficacy of Wild sirna for mutant allele:Poor efficacy
Relative difference :Moderate discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Ambion
Post-transfection duration: 24 hours
Concentration used:1.44mg
Reference:16113241
Delivery method:Transfection
Article title:Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo.
Authors:Holen T, Moe SE, Sørbø JG, Meza TJ, Ottersen OP, Klungland A.
Journal Reference:Nucleic Acids Res. 2005 Aug 19;33(15):4704-10. Print 2005.
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ASPsiRNA information siRNA Id:aspsirna1825
siRNA Name: siCD46
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Mismatch position in siRNA: U:A pair
Gene Information Gene Name Complement regulatory protein (CD46)
Target Sequence (5'->3'):GCCCCCAATATGTGAAAAG
Wild allele (5'->3'):NA
Mutant allele (5'->3'):CUUAUUGGAGAGAGCACGA
Position of siRNA on target gene: 779-797
Respective Gene/Protein Resources GenBank Accession:NM_002389.4
Cytogenic location:1q32.2
Chromosomal coordinates:1:207,925,382-207,968,860
UniProt ID:P15529
HUGO ID:6953
Reference SNp(RefSNP): rs12190959
Disease/Mutation information Target Mutation: c.104G>A, p.Cys35Tyr
Matation type/variant: single nucleotide variant/Missense variant
Mutation at gene level: NG_009296.1:g.9964G>A
Pathogenic status of mutation: Risk factor
Disease:Hemolytic uremic syndrome
Clinical Resources ClinVar ID:17049
KEGG disease ID:H00106, H0027
OMIM ID: 120920
COSMIC: CD46
DECIPHER: CD46
GeneTests: CD46
ASP siRNA details Mutant allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of ASP-siRNA for mutant allele:93
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GAAUAACCUCUCUCGUGCU
Percentage efficacy of Wild sirna for mutant allele:93
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:13nM
Reference:15781493
Delivery method:Transfection
Article title:A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites.
Authors:Du Q, Thonberg H, Wang J, Wahlestedt C, Liang Z.
Journal Reference:Nucleic Acids Res. 2005 Mar 21;33(5):1671-7. Print 2005. Erratum in: Nucleic Acids Res. 2005;33(11):3698.