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| ASPsiRNA information |
siRNA Id: | aspsirna1843
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|
siRNA Name: | siCD46
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
ASP-siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Mismatch position in siRNA: | G:G clash |
| Gene Information |
Gene Name | Complement regulatory protein (CD46) |
|
Target Sequence (5'->3'): | GCCCCCAATATGTGAAAAG
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
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Mutant allele (5'->3'): | CUUAUUGGAGAGAGCAGGA
|
|
Position of siRNA on target gene: | 779-797
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002389.4 |
| Cytogenic location: | 17p13 |
| Chromosomal coordinates: | 17:7,387,697-7,417,934 |
| UniProt ID: | P15529 |
| HUGO ID: | 6953 |
|
Reference SNp(RefSNP): | rs12190959 |
| Disease/Mutation information |
Target Mutation: | c.104G>A, p.Cys35Tyr
|
|
Matation type/variant: | single nucleotide variant/synonymous variant
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Mutation at gene level: | NG_009296.1:g.9964G>A
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Pathogenic status of mutation: | Risk factor
|
|
Disease: | Hemolytic uremic syndrome |
| Clinical Resources |
ClinVar ID: | 17049 |
|
KEGG disease ID: | H00106, H0027 |
|
OMIM ID: | 120920 |
|
COSMIC: | CD46 |
|
DECIPHER: | CD46 |
|
GeneTests: | CD46 |
| ASP siRNA details |
Mutant allele (5'->3'): | CUUAUUGGAGAGAGCAGGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 68
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 93
|
|
Relative difference: | 25
|
| Wild siRNA details |
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
Wild siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Percentage efficacy of wild siRNA for wild allele: | 93
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUAACCUCUCUCGUGCU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 68
|
|
Relative difference : | 25
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| Concentration used: | 13nM |
| Reference: | 15781493 |
| Delivery method: | Transfection |
'| Article title: | A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites. |
'| Authors: | Du Q, Thonberg H, Wang J, Wahlestedt C, Liang Z. |
'| Journal Reference: | Nucleic Acids Res. 2005 Mar 21;33(5):1671-7. Print 2005. Erratum in: Nucleic Acids Res. 2005;33(11):3698. |
'
