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| ASPsiRNA information |
siRNA Id: | aspsirna1864
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|
siRNA Name: | RMC845 dm
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCUGU
|
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ASP-siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCGGU
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|
Mismatch position in siRNA: | NA |
| Gene Information |
Gene Name | Aquaporin 4 (Aqp4) |
|
Target Sequence (5'->3'): | AACCGGAGCCAAGUGGAGCCA
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Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
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Mutant allele (5'->3'): | AACCGGAGCCAAGUGGAGCCA
|
|
Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_009700 |
| Cytogenic location: | 18q11.2 |
| Chromosomal coordinates: | 18:24,432,006-24,445,734 |
| UniProt ID: | P55087 |
| HUGO ID: | 637 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
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Matation type/variant: | NA
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Mutation at gene level: | NA
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Pathogenic status of mutation: | Mutation tolerance study
|
|
Disease: | Brain edema |
| Clinical Resources |
ClinVar ID: | NA |
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KEGG disease ID: | H00106 |
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OMIM ID: | 600308 |
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COSMIC: | Aqp4 |
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DECIPHER: | Aqp4 |
|
GeneTests: | Aqp4 |
| ASP siRNA details |
Mutant allele (5'->3'): | AACCGGAGCCAAGUGGAGCCA
|
|
ASP-siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCGGU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
|
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
ASP-siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCGGU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 15
|
|
Relative difference: | 55
|
| Wild siRNA details |
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
Wild siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCUGU
|
|
Percentage efficacy of wild siRNA for wild allele: | 58
|
|
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
ASP-siRNA (As strand 3'->5'): | UUGGCCUCGGUUCACCUCGGU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 75
|
|
Relative difference : | -17
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 24 hours |
| Concentration used: | 1.44mg |
| Reference: | 16113241 |
| Delivery method: | Transfection |
'| Article title: | Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo. |
'| Authors: | Holen T, Moe SE, Sørbø JG, Meza TJ, Ottersen OP, Klungland A. |
'| Journal Reference: | Nucleic Acids Res. 2005 Aug 19;33(15):4704-10. Print 2005. |
'
