| | |
ASPsiRNA information |
siRNA Id: | aspsirna1865
|
siRNA Name: | RMC466 w
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCACCU
|
ASP-siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCAUCU
|
Mismatch position in siRNA: | NA |
Gene Information |
Gene Name | Aquaporin 4 (Aqp4) |
Target Sequence (5'->3'): | UGGCCAUGGGCUCCUGGUAGA
|
Wild allele (5'->3'): | UGGCCAUGGGCUCCUGGUGGA
|
Mutant allele (5'->3'): | UGGCCAUGGGCUCCUGGUAGA
|
Position of siRNA on target gene: | NA
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_009700 |
Cytogenic location: | 18q11.2 |
Chromosomal coordinates: | 18:24,432,006-24,445,734 |
UniProt ID: | P55087 |
HUGO ID: | 637 |
Reference SNp(RefSNP): | NA |
Disease/Mutation information |
Target Mutation: | NA
|
Matation type/variant: | NA
|
Mutation at gene level: | NA
|
Pathogenic status of mutation: | Mutation tolerance study
|
Disease: | Brain edema |
Clinical Resources |
ClinVar ID: | NA |
KEGG disease ID: | H00106 |
OMIM ID: | 600308 |
COSMIC: | Aqp4 |
DECIPHER: | Aqp4 |
GeneTests: | Aqp4 |
ASP siRNA details |
Mutant allele (5'->3'): | UGGCCAUGGGCUCCUGGUAGA
|
ASP-siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCAUCU
|
Percentage efficacy of ASP-siRNA for mutant allele: | 10
|
Wild allele (5'->3'): | UGGCCAUGGGCUCCUGGUGGA
|
ASP-siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCAUCU
|
Percentage efficacy of ASP-siRNA for wild allele: | 42
|
Relative difference: | -32
|
Wild siRNA details |
Wild allele (5'->3'): | UGGCCAUGGGCUCCUGGUGGA
|
Wild siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCACCU
|
Percentage efficacy of wild siRNA for wild allele: | 77
|
Wild allele (5'->3'): | UGGCCAUGGGCUCCUGGUGGA
|
ASP-siRNA (As strand 3'->5'): | ACCGGUACCCGAGGACCAUCU
|
Percentage efficacy of Wild sirna for mutant allele: | 40
|
Relative difference : | 37
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HeLa |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Ambion |
Post-transfection duration: | 24 hours |
Concentration used: | 1.44mg |
Reference: | 16113241 |
Delivery method: | Transfection |
'Article title: | Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo. |
'Authors: | Holen T, Moe SE, Sørbø JG, Meza TJ, Ottersen OP, Klungland A. |
'Journal Reference: | Nucleic Acids Res. 2005 Aug 19;33(15):4704-10. Print 2005. |
'