aspsirna1871 details

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ASPsiRNA information siRNA Id:aspsirna1871
siRNA Name: RMC661 w
Mismatch information Wild siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACCCU
ASP-siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACUCU
Mismatch position in siRNA: NA
Gene Information Gene Name Aquaporin 4 (Aqp4)
Target Sequence (5'->3'):AGUUAUCAUGGGAAACUGAGA
Wild allele (5'->3'):AGUUAUCAUGGGAAACUGGGA
Mutant allele (5'->3'):AGUUAUCAUGGGAAACUGAGA
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_009700
Cytogenic location:18q11.2
Chromosomal coordinates:18:24,432,006-24,445,734
UniProt ID:P55087
HUGO ID:637
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: Mutation tolerance study
Disease:Brain edema
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00106
OMIM ID: 600308
COSMIC: Aqp4
DECIPHER: Aqp4
GeneTests: Aqp4
ASP siRNA details Mutant allele (5'->3'): AGUUAUCAUGGGAAACUGAGA
ASP-siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACUCU
Percentage efficacy of ASP-siRNA for mutant allele:26
Wild allele (5'->3'): AGUUAUCAUGGGAAACUGGGA
ASP-siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACUCU
Percentage efficacy of ASP-siRNA for wild allele:58
Relative difference:-32
Wild siRNA details Wild allele (5'->3'): AGUUAUCAUGGGAAACUGGGA
Wild siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACCCU
Percentage efficacy of wild siRNA for wild allele:75
Wild allele (5'->3'): AGUUAUCAUGGGAAACUGGGA
ASP-siRNA (As strand 3'->5'):UCAAUAGUACCCUUUGACUCU
Percentage efficacy of Wild sirna for mutant allele:5
Relative difference :70
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Ambion
Post-transfection duration: 24 hours
Concentration used:1.44mg
Reference:16113241
Delivery method:Transfection
Article title:Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo.
Authors:Holen T, Moe SE, Sørbø JG, Meza TJ, Ottersen OP, Klungland A.
Journal Reference:Nucleic Acids Res. 2005 Aug 19;33(15):4704-10. Print 2005.