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| ASPsiRNA information |
siRNA Id: | aspsirna1922
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siRNA Name: | si747/49_3D11
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
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ASP-siRNA (As strand 3'->5'): | AGUUCCUU---GUUGUAGAGG
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Mismatch position in siRNA: | DEL_12,13,14 |
| Gene Information |
Gene Name | epidermal growth factor receptor (EGFR) |
|
Target Sequence (5'->3'): | UCAAGGAACCAACAUCUCC
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Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
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Mutant allele (5'->3'): | UCAAGGAA----------CAACAUCUCC
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Position of siRNA on target gene: | 2477-2504
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_005228.3 |
| Cytogenic location: | 7p11.2 |
| Chromosomal coordinates: | 7:55,086,677-55,279,261 |
| UniProt ID: | P00533 |
| HUGO ID: | 3236 |
|
Reference SNp(RefSNP): | rs72750426 |
| Disease/Mutation information |
Target Mutation: | c.2235_2243delGGAATTAAG, p.Leu747_Glu749del
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Matation type/variant: | Deletion
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Mutation at gene level: | NG_007726.3:g.160741_160749delGGAATTAAG
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Pathogenic status of mutation: | Drug response
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 177663 |
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KEGG disease ID: | H00013 |
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OMIM ID: | 211980 |
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COSMIC: | EGFR |
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DECIPHER: | EGFR |
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GeneTests: | EGFR |
| ASP siRNA details |
Mutant allele (5'->3'): | UCAAGGAA----------CAACAUCUCC
|
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ASP-siRNA (As strand 3'->5'): | AGUUCCUU---GUUGUAGAGG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 60
|
|
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
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ASP-siRNA (As strand 3'->5'): | AGUUCCUU---GUUGUAGAGG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 5
|
|
Relative difference: | 55
|
| Wild siRNA details |
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
Wild siRNA (As strand 3'->5'): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
|
|
Percentage efficacy of wild siRNA for wild allele: | 59
|
|
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
ASP-siRNA (As strand 3'->5'): | AGUUCCUU---GUUGUAGAGG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 11
|
|
Relative difference : | 48
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Applied Biosystems |
| Post-transfection duration: | 24 hours |
| Concentration used: | 20nM |
| Reference: | 23951344 |
| Delivery method: | Atelocollagen mediated delivery |
'| Article title: | Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference. |
'| Authors: | Takahashi M, Chiyo T, Okada T, Hohjoh H. |
'| Journal Reference: | PLoS One. 2013 Aug 8;8(8):e73214. doi: 10.1371/journal.pone.0073214. eCollection 2013. |
'
