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| ASPsiRNA information |
siRNA Id: | aspsirna1923
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siRNA Name: | si747/49_3D3
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUUAAUUCUCUUCGUU
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ASP-siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUU----------GUU
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Mismatch position in siRNA: | DEL_4,5,6 |
| Gene Information |
Gene Name | epidermal growth factor receptor (EGFR) |
|
Target Sequence (5'->3'): | CGUCGCUAUCAAGGAACCA
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Wild allele (5'->3'): | CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
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Mutant allele (5'->3'): | CGUCGCUAUCAAGGAA----------CAA
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Position of siRNA on target gene: | 2469-2497
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_005228.3 |
| Cytogenic location: | 7p11.2 |
| Chromosomal coordinates: | 7:55,086,677-55,279,261 |
| UniProt ID: | P00533 |
| HUGO ID: | 3236 |
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Reference SNp(RefSNP): | rs12191322 |
| Disease/Mutation information |
Target Mutation: | c.2248G>C,p.A750P
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Matation type/variant: | single nucleotide variant/Missense variant
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Mutation at gene level: | NG_007726.3:g.160754G>C
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Pathogenic status of mutation: | Uncertain significance
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 45239 |
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KEGG disease ID: | H00013 |
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OMIM ID: | 211980 |
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COSMIC: | EGFR |
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DECIPHER: | EGFR |
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GeneTests: | EGFR |
| ASP siRNA details |
Mutant allele (5'->3'): | CGUCGCUAUCAAGGAA----------CAA
|
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ASP-siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUU----------GUU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 82
|
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Wild allele (5'->3'): | CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
|
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ASP-siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUU----------GUU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 10
|
|
Relative difference: | 72
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| Wild siRNA details |
Wild allele (5'->3'): | CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
|
|
Wild siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUUAAUUCUCUUCGUU
|
|
Percentage efficacy of wild siRNA for wild allele: | 59
|
|
Wild allele (5'->3'): | CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
|
|
ASP-siRNA (As strand 3'->5'): | GCAGCGAUAGUUCCUU----------GUU
|
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Percentage efficacy of Wild sirna for mutant allele: | 11
|
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Relative difference : | 48
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Applied Biosystems |
| Post-transfection duration: | 24 hours |
| Concentration used: | 20nM |
| Reference: | 23951344 |
| Delivery method: | Atelocollagen mediated delivery |
'| Article title: | Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference. |
'| Authors: | Takahashi M, Chiyo T, Okada T, Hohjoh H. |
'| Journal Reference: | PLoS One. 2013 Aug 8;8(8):e73214. doi: 10.1371/journal.pone.0073214. eCollection 2013. |
'
