aspsirna1927 details

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ASPsiRNA information siRNA Id:aspsirna1927
siRNA Name: si747/49_3D7
Mismatch information Wild siRNA (As strand 3'->5'):CGAUAGUUCCUUAAUUCUCUUCGUUGUA
ASP-siRNA (As strand 3'->5'):CGAUAGUUCCUU----------GUUGUA
Mismatch position in siRNA: DEL_8,9,10
Gene Information Gene Name epidermal growth factor receptor (EGFR)
Target Sequence (5'->3'):GCUAUCAAGGAACCAACAU
Wild allele (5'->3'):GCUAUCAAGGAAUUAAGAGAAGCAACAU
Mutant allele (5'->3'):GCUAUCAAGGAA----------CAACAU
Position of siRNA on target gene: 2473-2500
Respective Gene/Protein Resources GenBank Accession:NM_005228.3
Cytogenic location:7p11.2
Chromosomal coordinates:7:55,086,677-55,279,261
UniProt ID:P00533
HUGO ID:3236
Reference SNp(RefSNP): rs12191322
Disease/Mutation information Target Mutation: c.2248G>C,p.A750P
Matation type/variant: single nucleotide variant/Missense variant
Mutation at gene level: NG_007726.3:g.160754G>C
Pathogenic status of mutation: Uncertain significance
Disease:Cancer
Clinical Resources ClinVar ID:45239
KEGG disease ID:H00013
OMIM ID: 211980
COSMIC: EGFR
DECIPHER: EGFR
GeneTests: EGFR
ASP siRNA details Mutant allele (5'->3'): GCUAUCAAGGAA----------CAACAU
ASP-siRNA (As strand 3'->5'):CGAUAGUUCCUU----------GUUGUA
Percentage efficacy of ASP-siRNA for mutant allele:80
Wild allele (5'->3'): GCUAUCAAGGAAUUAAGAGAAGCAACAU
ASP-siRNA (As strand 3'->5'):CGAUAGUUCCUU----------GUUGUA
Percentage efficacy of ASP-siRNA for wild allele:22
Relative difference:58
Wild siRNA details Wild allele (5'->3'): GCUAUCAAGGAAUUAAGAGAAGCAACAU
Wild siRNA (As strand 3'->5'):CGAUAGUUCCUUAAUUCUCUUCGUUGUA
Percentage efficacy of wild siRNA for wild allele:59
Wild allele (5'->3'): GCUAUCAAGGAAUUAAGAGAAGCAACAU
ASP-siRNA (As strand 3'->5'):CGAUAGUUCCUU----------GUUGUA
Percentage efficacy of Wild sirna for mutant allele:11
Relative difference :48
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Applied Biosystems
Post-transfection duration: 24 hours
Concentration used:20nM
Reference:23951344
Delivery method:Atelocollagen mediated delivery
Article title:Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference.
Authors:Takahashi M, Chiyo T, Okada T, Hohjoh H.
Journal Reference:PLoS One. 2013 Aug 8;8(8):e73214. doi: 10.1371/journal.pone.0073214. eCollection 2013.