aspsirna1938 details

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ASPsiRNA information siRNA Id:aspsirna1938
siRNA Name: si746/50_3D16
Mismatch information Wild siRNA (As strand 3'->5'):GUUCCUUAAUUCUCUUCGUUGUAGAGGCUUUCGG
ASP-siRNA (As strand 3'->5'):GUU---------------UUGUAGAGGCUUUCGG
Mismatch position in siRNA: NA
Gene Information Gene Name epidermal growth factor receptor (EGFR)
Target Sequence (5'->3'):CAAAACAUCUCCGAAAGCC
Wild allele (5'->3'):CAAGGAAUUAAGAGAAGCAACAUCUCCGAAAGCC
Mutant allele (5'->3'):CAA---------------AACAUCUCCGAAAGCC
Position of siRNA on target gene: 2478-2511
Respective Gene/Protein Resources GenBank Accession:NM_005228.3
Cytogenic location:7p11.2
Chromosomal coordinates:7:55,086,677-55,279,261
UniProt ID:P00533
HUGO ID:3236
Reference SNp(RefSNP): rs12191342
Disease/Mutation information Target Mutation: c.2235_2249delGGAATTAAGAGAAGC, p.Glu746_Ala750del
Matation type/variant: Deletion/inframe variant
Mutation at gene level: NG_007726.3:g.160741_160755delGGAATTAAGAGAAGC
Pathogenic status of mutation: Drug response
Disease:Cancer
Clinical Resources ClinVar ID:163343
KEGG disease ID:H00013
OMIM ID: 211980
COSMIC: EGFR
DECIPHER: EGFR
GeneTests: EGFR
ASP siRNA details Mutant allele (5'->3'): CAA---------------AACAUCUCCGAAAGCC
ASP-siRNA (As strand 3'->5'):GUU---------------UUGUAGAGGCUUUCGG
Percentage efficacy of ASP-siRNA for mutant allele:70
Wild allele (5'->3'): CAAGGAAUUAAGAGAAGCAACAUCUCCGAAAGCC
ASP-siRNA (As strand 3'->5'):GUU---------------UUGUAGAGGCUUUCGG
Percentage efficacy of ASP-siRNA for wild allele:30
Relative difference:40
Wild siRNA details Wild allele (5'->3'): CAAGGAAUUAAGAGAAGCAACAUCUCCGAAAGCC
Wild siRNA (As strand 3'->5'):GUUCCUUAAUUCUCUUCGUUGUAGAGGCUUUCGG
Percentage efficacy of wild siRNA for wild allele:59
Wild allele (5'->3'): CAAGGAAUUAAGAGAAGCAACAUCUCCGAAAGCC
ASP-siRNA (As strand 3'->5'):GUU---------------UUGUAGAGGCUUUCGG
Percentage efficacy of Wild sirna for mutant allele:11
Relative difference :48
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Applied Biosystems
Post-transfection duration: 24 hours
Concentration used:20nM
Reference:23951344
Delivery method:Atelocollagen mediated delivery
Article title:Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference.
Authors:Takahashi M, Chiyo T, Okada T, Hohjoh H.
Journal Reference:PLoS One. 2013 Aug 8;8(8):e73214. doi: 10.1371/journal.pone.0073214. eCollection 2013.