aspsirna2017 details

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ASPsiRNA information siRNA Id:aspsirna2017
siRNA Name: Luc-5 siRNA-a13
Mismatch information Wild siRNA (As strand 3'->5'):AAAUACUCCUAGAGAGACU
ASP-siRNA (As strand 3'->5'):AAAUACACCUAGAGAGACU
Mismatch position in siRNA: A13U
Gene Information Gene Name P. luciferase
Target Sequence (5'->3'):CCCTTCTTGGCCTTTATGAGGATCTCTCTGATTT
Wild allele (5'->3'):UUUAUGAGGAUCUCUCUGA
Mutant allele (5'->3'):UUUAUGUGGAUCUCUCUGA
Position of siRNA on target gene: 2673-2691
Respective Gene/Protein Resources GenBank Accession:KP171169.1
Cytogenic location:NA
Chromosomal coordinates:NA
UniProt ID:P19840
HUGO ID:NA
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: P. luciferase
DECIPHER: P. luciferase
GeneTests: P. luciferase
ASP siRNA details Mutant allele (5'->3'): UUUAUGUGGAUCUCUCUGA
ASP-siRNA (As strand 3'->5'):AAAUACACCUAGAGAGACU
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): UUUAUGAGGAUCUCUCUGA
ASP-siRNA (As strand 3'->5'):AAAUACACCUAGAGAGACU
Percentage efficacy of ASP-siRNA for wild allele:30
Relative difference:60
Wild siRNA details Wild allele (5'->3'): UUUAUGAGGAUCUCUCUGA
Wild siRNA (As strand 3'->5'):AAAUACUCCUAGAGAGACU
Percentage efficacy of wild siRNA for wild allele:30
Wild allele (5'->3'): UUUAUGAGGAUCUCUCUGA
ASP-siRNA (As strand 3'->5'):AAAUACACCUAGAGAGACU
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:US2005/0176045A1
Delivery method:Transfection
Article title:SNP discriminatory siRNA
Authors:Yuriy Fedorov, Angela Reynolds, Anastasia Khvorova
Journal Reference:US20050176045 A1