| | |
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| ASPsiRNA information |
siRNA Id: | aspsirna2036
|
|
siRNA Name: | Luc-5 siRNA-g19
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | AAAUACUCCUAGAGAGACU
|
|
ASP-siRNA (As strand 3'->5'): | GAAUACUCCUAGAGAGACU
|
|
Mismatch position in siRNA: | G19A |
| Gene Information |
Gene Name | P. luciferase |
|
Target Sequence (5'->3'): | CCCTTCTTGGCCTTTATGAGGATCTCTCTGATTT
|
|
Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
|
|
Mutant allele (5'->3'): | CUUAUGAGGAUCUCUCUGA
|
|
Position of siRNA on target gene: | 2673-2691
|
| Respective Gene/Protein Resources |
GenBank Accession: | KP171169.1 |
| Cytogenic location: | NA |
| Chromosomal coordinates: | NA |
| UniProt ID: | P19840 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | NA
|
|
Mutation at gene level: | NA
|
|
Pathogenic status of mutation: | NA
|
|
Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
|
KEGG disease ID: | NA |
|
OMIM ID: | NA |
|
COSMIC: | P. luciferase |
|
DECIPHER: | P. luciferase |
|
GeneTests: | P. luciferase |
| ASP siRNA details |
Mutant allele (5'->3'): | CUUAUGAGGAUCUCUCUGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUACUCCUAGAGAGACU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 42
|
|
Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUACUCCUAGAGAGACU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 30
|
|
Relative difference: | 12
|
| Wild siRNA details |
Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
|
|
Wild siRNA (As strand 3'->5'): | AAAUACUCCUAGAGAGACU
|
|
Percentage efficacy of wild siRNA for wild allele: | 30
|
|
Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
|
|
ASP-siRNA (As strand 3'->5'): | GAAUACUCCUAGAGAGACU
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100nM |
| Reference: | US2005/0176045A1 |
| Delivery method: | Transfection |
'| Article title: | SNP discriminatory siRNA |
'| Authors: | Yuriy Fedorov, Angela Reynolds, Anastasia Khvorova |
'| Journal Reference: | US20050176045 A1 |
'
