aspsirna2084 details

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ASPsiRNA information siRNA Id:aspsirna2084
siRNA Name: hCyclo-siRNA-g16
Mismatch information Wild siRNA (As strand 3'->5'):GAGAGGACAUCGAUUCCGG
ASP-siRNA (As strand 3'->5'):GAGGGGACAUCGAUUCCGG
Mismatch position in siRNA: G16A
Gene Information Gene Name Peptidylprolyl isomerase B (PPIB)/Cyclophilin B
Target Sequence (5'->3'):NA
Wild allele (5'->3'):CUCUCCUGUAGCUAAGGCC
Mutant allele (5'->3'):CUCCCCUGUAGCUAAGGCC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_000942.4
Cytogenic location:15q22.31
Chromosomal coordinates:15:64,448,013-64,455,353
UniProt ID:Q13427
HUGO ID:9253
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00088
OMIM ID: 601753
COSMIC: PPIB/Cyclophilin B
DECIPHER: PPIB/Cyclophilin B
GeneTests: PPIB/Cyclophilin B
ASP siRNA details Mutant allele (5'->3'): CUCCCCUGUAGCUAAGGCC
ASP-siRNA (As strand 3'->5'):GAGGGGACAUCGAUUCCGG
Percentage efficacy of ASP-siRNA for mutant allele:58
Wild allele (5'->3'): CUCUCCUGUAGCUAAGGCC
ASP-siRNA (As strand 3'->5'):GAGGGGACAUCGAUUCCGG
Percentage efficacy of ASP-siRNA for wild allele:45
Relative difference:13
Wild siRNA details Wild allele (5'->3'): CUCUCCUGUAGCUAAGGCC
Wild siRNA (As strand 3'->5'):GAGAGGACAUCGAUUCCGG
Percentage efficacy of wild siRNA for wild allele:45
Wild allele (5'->3'): CUCUCCUGUAGCUAAGGCC
ASP-siRNA (As strand 3'->5'):GAGGGGACAUCGAUUCCGG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:US2005/0176045A1
Delivery method:Transfection
Article title:SNP discriminatory siRNA
Authors:Yuriy Fedorov, Angela Reynolds, Anastasia Khvorova
Journal Reference:US20050176045 A1