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| ASPsiRNA information |
siRNA Id: | aspsirna2113
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siRNA Name: | 25
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | ACCCGCAUCCGUUCUCACG
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|
ASP-siRNA (As strand 3'->5'): | AACCGCAUCCGUUCUCACG
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|
Mismatch position in siRNA: | A18C |
| Gene Information |
Gene Name | K-RAS (KRAS) |
|
Target Sequence (5'->3'): | gaatataaacttgtggtagttggagctggtggcgtaggcaagagtgccttgacgatacagctaattcagaatcattt
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|
Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
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Mutant allele (5'->3'): | UUGGCGUAGGCAAGAGUGC
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|
Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_033360.3 |
| Cytogenic location: | 12p12.1 |
| Chromosomal coordinates: | 12:25,358,179-25,403,869 |
| UniProt ID: | P01116 |
| HUGO ID: | 6407 |
|
Reference SNp(RefSNP): | rs12191352 |
| Disease/Mutation information |
Target Mutation: | c.35G>T,p.Gly12Val
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|
Matation type/variant: | single nucleotide variant/missense variant
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Mutation at gene level: | NG_007524.1:g.10571G>T
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Pathogenic status of mutation: | NA
|
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 12583 |
|
KEGG disease ID: | H00003 |
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OMIM ID: | 190070 |
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COSMIC: | KRAS |
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DECIPHER: | KRAS |
|
GeneTests: | KRAS |
| ASP siRNA details |
Mutant allele (5'->3'): | UUGGCGUAGGCAAGAGUGC
|
|
ASP-siRNA (As strand 3'->5'): | AACCGCAUCCGUUCUCACG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 3
|
|
Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
|
|
ASP-siRNA (As strand 3'->5'): | AACCGCAUCCGUUCUCACG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 15
|
|
Relative difference: | -12
|
| Wild siRNA details |
Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
|
|
Wild siRNA (As strand 3'->5'): | ACCCGCAUCCGUUCUCACG
|
|
Percentage efficacy of wild siRNA for wild allele: | 67
|
|
Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
|
|
ASP-siRNA (As strand 3'->5'): | AACCGCAUCCGUUCUCACG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 23
|
|
Relative difference : | 44
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| Concentration used: | 100nM |
| Reference: | US2005/0176045A1 |
| Delivery method: | Transfection |
'| Article title: | SNP discriminatory siRNA |
'| Authors: | Yuriy Fedorov, Angela Reynolds, Anastasia Khvorova |
'| Journal Reference: | US20050176045 A1 |
'
