aspsirna2134 details

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ASPsiRNA information siRNA Id:aspsirna2134
siRNA Name: 25
Mismatch information Wild siRNA (As strand 3'->5'):ACCCGCAUCCGUUCUCACG
ASP-siRNA (As strand 3'->5'):AACCGCAUCCGUUCUCACG
Mismatch position in siRNA: A18C
Gene Information Gene Name K-RAS (KRAS)
Target Sequence (5'->3'):gaatataaacttgtggtagttggagctggtggcgtaggcaagagtgccttgacgatacagctaattcagaatcattt
Wild allele (5'->3'):UGGGCGUAGGCAAGAGUGC
Mutant allele (5'->3'):UUGGCGUAGGCAAGAGUGC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_033360.3
Cytogenic location:12p12.1
Chromosomal coordinates:12:25,358,179-25,403,869
UniProt ID:P01116
HUGO ID:6407
Reference SNp(RefSNP): rs12191352
Disease/Mutation information Target Mutation: c.35G>T,p.Gly12Val
Matation type/variant: single nucleotide variant/missense variant
Mutation at gene level: NG_007524.1:g.10571G>T
Pathogenic status of mutation: NA
Disease:Cancer
Clinical Resources ClinVar ID:12583
KEGG disease ID:H00003
OMIM ID: 190070
COSMIC: KRAS
DECIPHER: KRAS
GeneTests: KRAS
ASP siRNA details Mutant allele (5'->3'): UUGGCGUAGGCAAGAGUGC
ASP-siRNA (As strand 3'->5'):AACCGCAUCCGUUCUCACG
Percentage efficacy of ASP-siRNA for mutant allele:3
Wild allele (5'->3'): UGGGCGUAGGCAAGAGUGC
ASP-siRNA (As strand 3'->5'):AACCGCAUCCGUUCUCACG
Percentage efficacy of ASP-siRNA for wild allele:15
Relative difference:-12
Wild siRNA details Wild allele (5'->3'): UGGGCGUAGGCAAGAGUGC
Wild siRNA (As strand 3'->5'):ACCCGCAUCCGUUCUCACG
Percentage efficacy of wild siRNA for wild allele:67
Wild allele (5'->3'): UGGGCGUAGGCAAGAGUGC
ASP-siRNA (As strand 3'->5'):AACCGCAUCCGUUCUCACG
Percentage efficacy of Wild sirna for mutant allele:23
Relative difference :44
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:US2005/0176045A1
Delivery method:Transfection
Article title:SNP discriminatory siRNA
Authors:Yuriy Fedorov, Angela Reynolds, Anastasia Khvorova
Journal Reference:US20050176045 A1