aspsirna2156 details

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ASPsiRNA information siRNA Id:aspsirna2156
siRNA Name:
Mismatch information Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCU
ASP-siRNA (As strand 3'->5'):GUCGACGUCGACGUCGUCU-VP
Mismatch position in siRNA: A9U,A15U
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
Wild allele (5'->3'):CAGCAGCAGCAGCAGCAGA
Mutant allele (5'->3'):CAGCUGCAGCUGCAGCAGU-VP
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_002111.7
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:P42858
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: microsatellite
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): CAGCUGCAGCUGCAGCAGU-VP
ASP-siRNA (As strand 3'->5'):GUCGACGUCGACGUCGUCU-VP
Percentage efficacy of ASP-siRNA for mutant allele:40
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGA
ASP-siRNA (As strand 3'->5'):GUCGACGUCGACGUCGUCU-VP
Percentage efficacy of ASP-siRNA for wild allele:50
Relative difference:-10
Wild siRNA details Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGA
Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCU
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGA
ASP-siRNA (As strand 3'->5'):GUCGACGUCGACGUCGUCU-VP
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: GM04281/GM04869
Experimental technique used: Western blot
Transfection method: RNAiMAX
siRNA expression method: Applied Biosystems
Post-transfection duration: 48 hours
Concentration used:25nM
Reference:24694346
Delivery method:Transfection
Article title:Exploring the effect of sequence length and composition on allele-selective inhibition of human huntingtin expression by single-stranded silencing RNAs.
Authors:Hu J, Liu J, Yu D, Aiba Y, Lee S, Pendergraff H, Boubaker J, Artates JW, Lagier-Tourenne C, Lima WF, Swayze EE, Prakash TP, Corey DR.
Journal Reference:Nucleic Acid Ther. 2014 Jun;24(3):199-209. doi: 10.1089/nat.2013.0476. Epub 2014 Apr 2