| | |
ASPsiRNA information |
siRNA Id: | aspsirna2172
|
siRNA Name: | sh39Cp16
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | CAUUAAGUUGCGAUCUUCUA
|
ASP-siRNA (As strand 3'->5'): | CAUGAAGUUGCGAUCUUCUA
|
Mismatch position in siRNA: | G16U |
Gene Information |
Gene Name | Hungtintin (HTT) |
Target Sequence (5'->3'): | AGCTTTGATGGATTCTAA
|
Wild allele (5'->3'): | GUAAUUCAACGCUAGAAGAU
|
Mutant allele (5'->3'): | GUACUUCAACGCUAGAAGAU
|
Position of siRNA on target gene: | 5478-5494
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
Cytogenic location: | 4p16.3 |
Chromosomal coordinates: | 4:3,076,407-3,245,686 |
UniProt ID: | P42858 |
HUGO ID: | 4851 |
Reference SNp(RefSNP): | rs19392295 |
Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
Matation type/variant: | microsatellite
|
Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Huntington disease (HD) |
Clinical Resources |
ClinVar ID: | 31916 |
KEGG disease ID: | H00059 |
OMIM ID: | 143100 |
COSMIC: | HTT |
DECIPHER: | HTT |
GeneTests: | HTT |
ASP siRNA details |
Mutant allele (5'->3'): | GUACUUCAACGCUAGAAGAU
|
ASP-siRNA (As strand 3'->5'): | CAUGAAGUUGCGAUCUUCUA
|
Percentage efficacy of ASP-siRNA for mutant allele: | 88
|
Wild allele (5'->3'): | GUAAUUCAACGCUAGAAGAU
|
ASP-siRNA (As strand 3'->5'): | CAUGAAGUUGCGAUCUUCUA
|
Percentage efficacy of ASP-siRNA for wild allele: | 15
|
Relative difference: | 73
|
Wild siRNA details |
Wild allele (5'->3'): | GUAAUUCAACGCUAGAAGAU
|
Wild siRNA (As strand 3'->5'): | CAUUAAGUUGCGAUCUUCUA
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
Wild allele (5'->3'): | GUAAUUCAACGCUAGAAGAU
|
ASP-siRNA (As strand 3'->5'): | CAUGAAGUUGCGAUCUUCUA
|
Percentage efficacy of Wild sirna for mutant allele: | 10
|
Relative difference : | 80
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HEK293 |
Experimental technique used: | RT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Invitrogen |
Post-transfection duration: | 48 hours |
Concentration used: | 6nM |
Reference: | 24926995 |
Delivery method: | Lentiviral vector delivery |
'Article title: | Allele-specific silencing of mutant huntingtin in rodent brain and human stem cells. |
'Authors: | Drouet V, Ruiz M, Zala D, Feyeux M, Auregan G, Cambon K, Troquier L, Carpentier J, Aubert S, Merienne N, Bourgois-Rocha F, Hassig R, Rey M, Dufour N, Saudou F, Perrier AL, Hantraye P, Déglon N. |
'Journal Reference: | PLoS One. 2014 Jun 13;9(6):e99341. doi: 10.1371/journal.pone.0099341. eCollection 2014 |
'