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| ASPsiRNA information |
siRNA Id: | aspsirna2201
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siRNA Name: | PM4
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCG
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUAAAAGUCGUCG
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Mismatch position in siRNA: | A8C,A9U,A10G,A11C |
| Gene Information |
Gene Name | Atrophin-1 (ATN-1) |
|
Target Sequence (5'->3'): | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCACG
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Mutant allele (5'->3'): | CAGCAGCAUUUUCAGCAGC
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Position of siRNA on target gene: | 1699-1717,1720-1738
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_001007026.1 |
| Cytogenic location: | 12p13.31 |
| Chromosomal coordinates: | 12:7,033,625-7,051,483 |
| UniProt ID: | P54259 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | rs19392293 |
| Disease/Mutation information |
Target Mutation: | c.1462_1464CAG(90_93)
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Matation type/variant: | STR short tandem repeat (microsatellite) variation
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Mutation at gene level: | NG_008047.1:g.17267_17269CAG(90_93)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Dentatorubral-pallidoluysian atrophy (DRPLA) |
| Clinical Resources |
ClinVar ID: | 38905 |
|
KEGG disease ID: | K05626 |
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OMIM ID: | 125370 |
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COSMIC: | ATN-1 |
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DECIPHER: | ATN-1 |
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GeneTests: | ATN-1 |
| ASP siRNA details |
Mutant allele (5'->3'): | CAGCAGCAUUUUCAGCAGC
|
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUAAAAGUCGUCG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 80
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUAAAAGUCGUCG
|
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Percentage efficacy of ASP-siRNA for wild allele: | 30
|
|
Relative difference: | 50
|
| Wild siRNA details |
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
|
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCG
|
|
Percentage efficacy of wild siRNA for wild allele: | 25
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUAAAAGUCGUCG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 20
|
|
Relative difference : | 5
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Isis Pharmaceuticals |
| Post-transfection duration: | 48 hours |
| Concentration used: | 50nM |
| Reference: | 24981774 |
| Delivery method: | Transfection |
'| Article title: | Allele-selective inhibition of mutant atrophin-1 expression by duplex and single-stranded RNAs. |
'| Authors: | Hu J, Liu J, Narayanannair KJ, Lackey JG, Kuchimanchi S, Rajeev KG, Manoharan M, Swayze EE, Lima WF, Prakash TP, Xiang Q, Martinez C, Corey DR |
'| Journal Reference: | Biochemistry. 2014 Jul 22;53(28):4510-8. doi: 10.1021/bi500610r. Epub 2014 Jul 11 |
'
