aspsirna2253 details

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ASPsiRNA information siRNA Id:aspsirna2253
siRNA Name: P6
Mismatch information Wild siRNA (As strand 3'->5'):ACAACCUCUGAACCCGUUA
ASP-siRNA (As strand 3'->5'):ACAACCUCUGAACGCGUUA
Mismatch position in siRNA: G6C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):AGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAGAUGGU
Wild allele (5'->3'):UGUUGGAGACUUGGGCAAU
Mutant allele (5'->3'):UGUUGGAGACUUGCGCAAU
Position of siRNA on target gene: 391-409
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.11
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.256G>C,p.Gly86Arg
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12653G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14758
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): UGUUGGAGACUUGCGCAAU
ASP-siRNA (As strand 3'->5'):ACAACCUCUGAACGCGUUA
Percentage efficacy of ASP-siRNA for mutant allele:90
Wild allele (5'->3'): UGUUGGAGACUUGGGCAAU
ASP-siRNA (As strand 3'->5'):ACAACCUCUGAACGCGUUA
Percentage efficacy of ASP-siRNA for wild allele:70
Relative difference:20
Wild siRNA details Wild allele (5'->3'): UGUUGGAGACUUGGGCAAU
Wild siRNA (As strand 3'->5'):ACAACCUCUGAACCCGUUA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UGUUGGAGACUUGGGCAAU
ASP-siRNA (As strand 3'->5'):ACAACCUCUGAACGCGUUA
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :58
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Drosophila embryo lysate
Experimental technique used: In vitro cleavage assay
Transfection method: Lipofectamine 2000
siRNA expression method: Chemically synthesized
Post-transfection duration: 2 hours
Concentration used:50nM
Reference:US2007/0259827A1
Delivery method:Transfection
Article title:Compositions And Methods For Enhancing Discriminatory Rna Interference
Authors:Neil Aronin, Dianne Schwarz, Phillip D. Zamore
Journal Reference:US2007/0259827A1