| | |
ASPsiRNA information |
siRNA Id: | aspsirna2320
|
siRNA Name: | P6
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | ACAACCUCUGAACCCGUUA
|
ASP-siRNA (As strand 3'->5'): | ACAACCUCUGAACGCGUUA
|
Mismatch position in siRNA: | G6C |
Gene Information |
Gene Name | Cu,Zn superoxide dismutase (SOD1) |
Target Sequence (5'->3'): | AGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAGAUGGU
|
Wild allele (5'->3'): | UGUUGGAGACUUGGGCAAU
|
Mutant allele (5'->3'): | UGUUGGAGACUUGCGCAAU
|
Position of siRNA on target gene: | 391-409
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000454.4 |
Cytogenic location: | 21q22.11 |
Chromosomal coordinates: | 21:33,031,934-33,041,243 |
UniProt ID: | P00441 |
HUGO ID: | 11179 |
Reference SNp(RefSNP): | rs12191243 |
Disease/Mutation information |
Target Mutation: | c.256G>C,p.Gly86Arg
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_008689.1:g.12653G>C
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Amyotrophic Lateral Sclerosis (ALS) |
Clinical Resources |
ClinVar ID: | 14758 |
KEGG disease ID: | H00058 |
OMIM ID: | 105400 |
COSMIC: | SOD1 |
DECIPHER: | SOD1 |
GeneTests: | SOD1 |
ASP siRNA details |
Mutant allele (5'->3'): | UGUUGGAGACUUGCGCAAU
|
ASP-siRNA (As strand 3'->5'): | ACAACCUCUGAACGCGUUA
|
Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
Wild allele (5'->3'): | UGUUGGAGACUUGGGCAAU
|
ASP-siRNA (As strand 3'->5'): | ACAACCUCUGAACGCGUUA
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
Relative difference: | 9
|
Wild siRNA details |
Wild allele (5'->3'): | UGUUGGAGACUUGGGCAAU
|
Wild siRNA (As strand 3'->5'): | ACAACCUCUGAACCCGUUA
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | UGUUGGAGACUUGGGCAAU
|
ASP-siRNA (As strand 3'->5'): | ACAACCUCUGAACGCGUUA
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
Relative difference : | 47
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HeLa |
Experimental technique used: | RT-PCR |
Transfection method: | OligofectAMINE |
siRNA expression method: | Chemically synthesized |
Post-transfection duration: | 24 hours |
Concentration used: | 100nM |
Reference: | US2007/0259827A1 |
Delivery method: | Transfection |
'Article title: | Compositions And Methods For Enhancing Discriminatory Rna Interference |
'Authors: | Neil Aronin, Dianne Schwarz, Phillip D. Zamore |
'Journal Reference: | US2007/0259827A1 |
'