| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna2450
|
|
siRNA Name: | 45
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | UUGAGGAGGCGCGUUGAAA
|
|
ASP-siRNA (As strand 3'->5'): | UUGAUGAGGCGCGUUGAAA
|
|
Mismatch position in siRNA: | U15G |
| Gene Information |
Gene Name | K-RAS (KRAS) |
|
Target Sequence (5'->3'): | GAGGATGCTTTTTATACATTGGTGAGGGAGATCCGACAATAC
|
|
Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
|
|
Mutant allele (5'->3'): | AACUACUCCGCGCAACUUU
|
|
Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_033360.3 |
| Cytogenic location: | 12p12.1 |
| Chromosomal coordinates: | 12:25,358,179-25,403,869 |
| UniProt ID: | P01116 |
| HUGO ID: | 6407 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | NA
|
|
Mutation at gene level: | NA
|
|
Pathogenic status of mutation: | NA
|
|
Disease: | NA |
| Clinical Resources |
ClinVar ID: | NA |
|
OMIM ID: | 190070 |
|
COSMIC: | KRAS |
|
DECIPHER: | KRAS |
|
GeneTests: | KRAS |
| ASP siRNA details |
Mutant allele (5'->3'): | AACUACUCCGCGCAACUUU
|
|
ASP-siRNA (As strand 3'->5'): | UUGAUGAGGCGCGUUGAAA
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 10
|
|
Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
|
|
ASP-siRNA (As strand 3'->5'): | UUGAUGAGGCGCGUUGAAA
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 70
|
|
Relative difference: | -60
|
| Wild siRNA details |
Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
|
|
Wild siRNA (As strand 3'->5'): | UUGAGGAGGCGCGUUGAAA
|
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
|
Wild allele (5'->3'): | AACUCCUCCGCGCAACUUU
|
|
ASP-siRNA (As strand 3'->5'): | UUGAUGAGGCGCGUUGAAA
|
|
Percentage efficacy of Wild sirna for mutant allele: | 50
|
|
Relative difference : | 20
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| Concentration used: | 100nM |
| Reference: | US007576197B2 |
| Delivery method: | Transfection |
'| Article title: | SiRNA targeting KRAS |
'| Authors: | Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Stephen Scaringe,Steven Read |
'| Journal Reference: | US007576197B2 |
'
