aspsirna2450 details

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ASPsiRNA information siRNA Id:aspsirna2450
siRNA Name: 45
Mismatch information Wild siRNA (As strand 3'->5'):UUGAGGAGGCGCGUUGAAA
ASP-siRNA (As strand 3'->5'):UUGAUGAGGCGCGUUGAAA
Mismatch position in siRNA: U15G
Gene Information Gene Name K-RAS (KRAS)
Target Sequence (5'->3'):GAGGATGCTTTTTATACATTGGTGAGGGAGATCCGACAATAC
Wild allele (5'->3'):AACUCCUCCGCGCAACUUU
Mutant allele (5'->3'):AACUACUCCGCGCAACUUU
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_033360.3
Cytogenic location:12p12.1
Chromosomal coordinates:12:25,358,179-25,403,869
UniProt ID:P01116
HUGO ID:6407
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
OMIM ID: 190070
COSMIC: KRAS
DECIPHER: KRAS
GeneTests: KRAS
ASP siRNA details Mutant allele (5'->3'): AACUACUCCGCGCAACUUU
ASP-siRNA (As strand 3'->5'):UUGAUGAGGCGCGUUGAAA
Percentage efficacy of ASP-siRNA for mutant allele:10
Wild allele (5'->3'): AACUCCUCCGCGCAACUUU
ASP-siRNA (As strand 3'->5'):UUGAUGAGGCGCGUUGAAA
Percentage efficacy of ASP-siRNA for wild allele:70
Relative difference:-60
Wild siRNA details Wild allele (5'->3'): AACUCCUCCGCGCAACUUU
Wild siRNA (As strand 3'->5'):UUGAGGAGGCGCGUUGAAA
Percentage efficacy of wild siRNA for wild allele:70
Wild allele (5'->3'): AACUCCUCCGCGCAACUUU
ASP-siRNA (As strand 3'->5'):UUGAUGAGGCGCGUUGAAA
Percentage efficacy of Wild sirna for mutant allele:50
Relative difference :20
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:US007576197B2
Delivery method:Transfection
Article title:SiRNA targeting KRAS
Authors:Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Stephen Scaringe,Steven Read
Journal Reference:US007576197B2