| | |
ASPsiRNA information |
siRNA Id: | aspsirna2531
|
siRNA Name: | SEQ ID NO 100
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
ASP-siRNA (As strand 3'->5'): | CUCCUUUCUCGUAGAUGCC
|
Mismatch position in siRNA: | NA |
Gene Information |
Gene Name | Peptidylprolyl isomerase B (PPIB)/Cyclophilin B |
Target Sequence (5'->3'): | gttccaaaaacagtggataattttgtggccttagctacaggagagaaaggatttggctacaaaaacagcaaattccatcgtgtaatcaa
|
Wild allele (5'->3'): | NA
|
Mutant allele (5'->3'): | GAGGAAAGAGCAUCUACGG
|
Position of siRNA on target gene: | 510-528
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000942 |
Cytogenic location: | 15q22.31 |
Chromosomal coordinates: | 15:64,448,013-64,455,353 |
UniProt ID: | Q13427 |
HUGO ID: | 9255 |
Reference SNp(RefSNP): | NA |
Disease/Mutation information |
Target Mutation: | NA
|
Matation type/variant: | NA
|
Mutation at gene level: | NA
|
Pathogenic status of mutation: | NA
|
Disease: | NA |
Clinical Resources |
ClinVar ID: | NA |
KEGG disease ID: | NA |
OMIM ID: | 123841 |
COSMIC: | PPIB/Cyclophilin B |
DECIPHER: | PPIB/Cyclophilin B |
GeneTests: | PPIB/Cyclophilin B |
ASP siRNA details |
Mutant allele (5'->3'): | GAGGAAAGAGCAUCUACGG
|
ASP-siRNA (As strand 3'->5'): | CUCCUUUCUCGUAGAUGCC
|
Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | CUCCUUUCUCGUAGAUGCC
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
Relative difference: | 33
|
Wild siRNA details |
Wild allele (5'->3'): | NA
|
Wild siRNA (As strand 3'->5'): | NA
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | CUCCUUUCUCGUAGAUGCC
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
Relative difference : | NA
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HEK293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 24 hours |
Concentration used: | 100nM |
Reference: | US007576197B2 |
Delivery method: | Transfection |
'Article title: | SiRNA targeting KRAS |
'Authors: | Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Stephen Scaringe,Steven Read |
'Journal Reference: | US007576197B2 |
'