aspsirna2678 details

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ASPsiRNA information siRNA Id:aspsirna2678
siRNA Name: SEQ ID NO 157
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):CUUUCCCUGAAGGUUCCUU
Mismatch position in siRNA: NA
Gene Information Gene Name Diazepam binding inhibitor (DBI)(GABA receptor modulator, acyl-CoA binding protein)
Target Sequence (5'->3'):acgggcaaggccaagtgggatgcctggaatgagctgaaagggacttccaaggaagatgccatgaaagcttacatcaacaaagtagaaga
Wild allele (5'->3'):NA
Mutant allele (5'->3'):GAAAGGGACUUCCAAGGAA
Position of siRNA on target gene: 425-443
Respective Gene/Protein Resources GenBank Accession:NM_020548.7
Cytogenic location:2q14.2
Chromosomal coordinates:2:120,124,503-120,130,121
UniProt ID:P07108
HUGO ID:2690
Reference SNp(RefSNP): rs2276596
Disease/Mutation information Target Mutation: c.178 plus 29C>A
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NA
Pathogenic status of mutation: Pathogenic
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: 125950
COSMIC: DBI
DECIPHER: DBI
GeneTests: DBI
ASP siRNA details Mutant allele (5'->3'): GAAAGGGACUUCCAAGGAA
ASP-siRNA (As strand 3'->5'):CUUUCCCUGAAGGUUCCUU
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CUUUCCCUGAAGGUUCCUU
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:67
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):CUUUCCCUGAAGGUUCCUU
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: DU145
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:US007576197B2
Delivery method:Transfection
Article title:SiRNA targeting KRAS
Authors:Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Stephen Scaringe,Steven Read
Journal Reference:US007576197B2