aspsirna2867 details

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ASPsiRNA information siRNA Id:aspsirna2867
siRNA Name: SEQ ID NO 256
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):GCGCUUUUUCAACGCGCCU
Mismatch position in siRNA: NA
Gene Information Gene Name Firefly luciferase
Target Sequence (5'->3'):tgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaa
Wild allele (5'->3'):NA
Mutant allele (5'->3'):CGCGAAAAAGUUGCGCGGA
Position of siRNA on target gene: 5387-5405
Respective Gene/Protein Resources GenBank Accession:JN542719.1
Cytogenic location:NA
Chromosomal coordinates:NA
UniProt ID:Q27758
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: NA
Disease:NA
Clinical Resources ClinVar ID:NA
KEGG disease ID:NA
OMIM ID: NA
COSMIC: Firefly luciferase
DECIPHER: Firefly luciferase
GeneTests: Firefly luciferase
ASP siRNA details Mutant allele (5'->3'): CGCGAAAAAGUUGCGCGGA
ASP-siRNA (As strand 3'->5'):GCGCUUUUUCAACGCGCCU
Percentage efficacy of ASP-siRNA for mutant allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GCGCUUUUUCAACGCGCCU
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:70
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GCGCUUUUUCAACGCGCCU
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
Concentration used:100nM
Reference:US007576197B2
Delivery method:Transfection
Article title:SiRNA targeting KRAS
Authors:Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Stephen Scaringe,Steven Read
Journal Reference:US007576197B2