aspsirna3006 details

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ASPsiRNA information siRNA Id:aspsirna3006
siRNA Name: siSNCA_T1-5(10A)
Mismatch information Wild siRNA (As strand 3'->5'):AAGAUUUUCAGUGAUCAUC
ASP-siRNA (As strand 3'->5'):AAGAUUUUCUGUGAUCAUC
Mismatch position in siRNA: U10A
Gene Information Gene Name Synuclein, alpha (SNCA)
Target Sequence (5'->3'):TCGAGTGGGGCATAGTCATTTCTAAAAGTCACTAGTAGAAAGTATAATTTCAAGATTACTAGT
Wild allele (5'->3'):UUCUAAAAGUCACUAGUAG
Mutant allele (5'->3'):UUCUAAAAGACACUAGUAG
Position of siRNA on target gene: 2072-2090
Respective Gene/Protein Resources GenBank Accession:NM_001146055.1
Cytogenic location:4q22.1
Chromosomal coordinates:4:90,645,249-90,759,446
UniProt ID:P37840
HUGO ID:11138
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: SNCA locus triplication
Matation type/variant: Copy number gain
Mutation at gene level: NA
Pathogenic status of mutation: Pathogenic
Disease:Parkinsons disease (PD)
Clinical Resources ClinVar ID:14009
KEGG disease ID:H00057
OMIM ID: 168600
COSMIC: SNCA
DECIPHER: SNCA
GeneTests: SNCA
ASP siRNA details Mutant allele (5'->3'): UUCUAAAAGACACUAGUAG
ASP-siRNA (As strand 3'->5'):AAGAUUUUCUGUGAUCAUC
Percentage efficacy of ASP-siRNA for mutant allele:80
Wild allele (5'->3'): UUCUAAAAGUCACUAGUAG
ASP-siRNA (As strand 3'->5'):AAGAUUUUCUGUGAUCAUC
Percentage efficacy of ASP-siRNA for wild allele:20
Relative difference:60
Wild siRNA details Wild allele (5'->3'): UUCUAAAAGUCACUAGUAG
Wild siRNA (As strand 3'->5'):AAGAUUUUCAGUGAUCAUC
Percentage efficacy of wild siRNA for wild allele:85
Wild allele (5'->3'): UUCUAAAAGUCACUAGUAG
ASP-siRNA (As strand 3'->5'):AAGAUUUUCUGUGAUCAUC
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :65
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: S2
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine2000
siRNA expression method: Sigma-Aldrich
Post-transfection duration: 24 hours
Concentration used:20 nmol
Reference:25965551
Delivery method:Transfection
Article title:Normalization of Overexpressed ?-Synuclein Causing Parkinson’s Disease By a Moderate Gene Silencing With RNA Interference
Authors:Takahashi M, Suzuki M, Fukuoka M, Fujikake N, Watanabe S, Murata M, Wada K, Nagai Y, Hohjoh H
Journal Reference:Mol Ther Nucleic Acids. 2015 May 12;4:e241. doi: 10.1038/mtna.2015.14