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| ASPsiRNA information |
siRNA Id: | aspsirna3006
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siRNA Name: | siSNCA_T1-5(10A)
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | AAGAUUUUCAGUGAUCAUC
|
|
ASP-siRNA (As strand 3'->5'): | AAGAUUUUCUGUGAUCAUC
|
|
Mismatch position in siRNA: | U10A |
| Gene Information |
Gene Name | Synuclein, alpha (SNCA) |
|
Target Sequence (5'->3'): | TCGAGTGGGGCATAGTCATTTCTAAAAGTCACTAGTAGAAAGTATAATTTCAAGATTACTAGT
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|
Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
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Mutant allele (5'->3'): | UUCUAAAAGACACUAGUAG
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|
Position of siRNA on target gene: | 2072-2090
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_001146055.1 |
| Cytogenic location: | 4q22.1 |
| Chromosomal coordinates: | 4:90,645,249-90,759,446 |
| UniProt ID: | P37840 |
| HUGO ID: | 11138 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | SNCA locus triplication
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Matation type/variant: | Copy number gain
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Mutation at gene level: | NA
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Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 14009 |
|
KEGG disease ID: | H00057 |
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OMIM ID: | 168600 |
|
COSMIC: | SNCA |
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DECIPHER: | SNCA |
|
GeneTests: | SNCA |
| ASP siRNA details |
Mutant allele (5'->3'): | UUCUAAAAGACACUAGUAG
|
|
ASP-siRNA (As strand 3'->5'): | AAGAUUUUCUGUGAUCAUC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 80
|
|
Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
|
|
ASP-siRNA (As strand 3'->5'): | AAGAUUUUCUGUGAUCAUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 20
|
|
Relative difference: | 60
|
| Wild siRNA details |
Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
|
|
Wild siRNA (As strand 3'->5'): | AAGAUUUUCAGUGAUCAUC
|
|
Percentage efficacy of wild siRNA for wild allele: | 85
|
|
Wild allele (5'->3'): | UUCUAAAAGUCACUAGUAG
|
|
ASP-siRNA (As strand 3'->5'): | AAGAUUUUCUGUGAUCAUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 20
|
|
Relative difference : | 65
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | S2 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine2000 |
| siRNA expression method: | Sigma-Aldrich |
| Post-transfection duration: | 24 hours |
| Concentration used: | 20 nmol |
| Reference: | 25965551 |
| Delivery method: | Transfection |
'| Article title: | Normalization of Overexpressed ?-Synuclein Causing Parkinson’s Disease By a Moderate Gene Silencing With RNA Interference |
'| Authors: | Takahashi M, Suzuki M, Fukuoka M, Fujikake N, Watanabe S, Murata M, Wada K, Nagai Y, Hohjoh H |
'| Journal Reference: | Mol Ther Nucleic Acids. 2015 May 12;4:e241. doi: 10.1038/mtna.2015.14 |
'
