| | |
ASPsiRNA information |
siRNA Id: | aspsirna3028
|
siRNA Name: | P5
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCG
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUAGUCG
|
Mismatch position in siRNA: | A5C |
Gene Information |
Gene Name | Ataxin-3 (ATXN3) |
Target Sequence (5'->3'): | CAGCAGCAGCAGCAGCAGCAGCAGGGGGACCTATCAGGACAGAGTT
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
Mutant allele (5'->3'): | CAGCAGCAGCAGCAUCAGC
|
Position of siRNA on target gene: | 961-979
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_004993.5 |
Cytogenic location: | 14q32.12 |
Chromosomal coordinates: | 14:92,524,895-92,572,964 |
UniProt ID: | P54252 |
HUGO ID: | 7106 |
Reference SNp(RefSNP): | rs19392292 |
Disease/Mutation information |
Target Mutation: | c.892_894CAG(8_36) (p.Gln298_Gln305=)
|
Matation type/variant: | Microsatellite/synonymous variant
|
Mutation at gene level: | NG_008198.2:g.40588_40590CAG(8_36)
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Machado-Joseph Disease (MJD)/Spinocerebellar ataxia type 3 (SCA3) |
Clinical Resources |
ClinVar ID: | 3551 |
KEGG disease ID: | H00063 |
OMIM ID: | 109150 |
COSMIC: | ATXN3 |
DECIPHER: | ATXN3 |
GeneTests: | ATXN3 |
ASP siRNA details |
Mutant allele (5'->3'): | CAGCAGCAGCAGCAUCAGC
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUAGUCG
|
Percentage efficacy of ASP-siRNA for mutant allele: | 60
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUAGUCG
|
Percentage efficacy of ASP-siRNA for wild allele: | 50
|
Relative difference: | 10
|
Wild siRNA details |
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCG
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUAGUCG
|
Percentage efficacy of Wild sirna for mutant allele: | 50
|
Relative difference : | 20
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | Fibroblast |
Experimental technique used: | Western blot |
Transfection method: | RNAiMAX |
siRNA expression method: | Integrated DNA Technologies |
Post-transfection duration: | 24 hours |
Concentration used: | 12nM |
Reference: | 21294677 |
Delivery method: | Transfection |
'Article title: | Allele-selective inhibition of ataxin-3 (ATX3) expression by antisense oligomers and duplex RNAs |
'Authors: | Hu J, Gagnon KT, Liu J, Watts JK, Syeda-Nawaz J, Bennett CF, Swayze EE, Randolph J, Chattopadhyaya J, Corey DR |
'Journal Reference: | Biol Chem. 2011 Apr;392(4):315-25. doi: 10.1515/BC.2011.045. Epub 2011 Feb 7 |
'