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| ASPsiRNA information |
siRNA Id: | aspsirna3075
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siRNA Name: | AR-mt1-siRNA
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CGUACUUGGCCUCCGGGUA
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ASP-siRNA (As strand 3'->5'): | CGUACUUGACCUCCGGGUAGG
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|
Mismatch position in siRNA: | A13G |
| Gene Information |
Gene Name | Tumor protein p53 |
|
Target Sequence (5'->3'): | GCATGAACCGGAGGCCCATCCTCACCATCATCA
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Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
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Mutant allele (5'->3'): | GCAUGAACUGGAGGCCCAUCC
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|
Position of siRNA on target gene: | 936-954
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000546 |
| Cytogenic location: | 17p13.1 |
| Chromosomal coordinates: | 17:7,668,401-7,687,549 |
| UniProt ID: | P04637 |
| HUGO ID: | 11998 |
|
Reference SNp(RefSNP): | rs58778208 |
| Disease/Mutation information |
Target Mutation: | c.944C>T,p.R249W
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_017013.2:g.18333C>T
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Pathogenic status of mutation: | Pathogenic
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 141881 |
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KEGG disease ID: | H00881 |
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OMIM ID: | 191170 |
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COSMIC: | TP53 |
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DECIPHER: | TP53 |
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GeneTests: | TP53 |
| ASP siRNA details |
Mutant allele (5'->3'): | GCAUGAACUGGAGGCCCAUCC
|
|
ASP-siRNA (As strand 3'->5'): | CGUACUUGACCUCCGGGUAGG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 75
|
|
Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
|
|
ASP-siRNA (As strand 3'->5'): | CGUACUUGACCUCCGGGUAGG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 60
|
|
Relative difference: | 15
|
| Wild siRNA details |
Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
|
|
Wild siRNA (As strand 3'->5'): | CGUACUUGGCCUCCGGGUA
|
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
|
Wild allele (5'->3'): | GCAUGAACCGGAGGCCCAU
|
|
ASP-siRNA (As strand 3'->5'): | CGUACUUGACCUCCGGGUAGG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 60
|
|
Relative difference : | 10
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | H1299 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine |
| siRNA expression method: | MWG Biotech |
| Post-transfection duration: | 24 hours |
| Concentration used: | 400 ng |
| Reference: | 12403821 |
| Delivery method: | shRNA vector mediated delivery |
'| Article title: | Synthetic small inhibiting RNAs: Efficient tools to inactivate oncogenic mutations and restore p53 pathways |
'| Authors: | Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A |
'| Journal Reference: | Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28 |
'
