aspsirna3075 details

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ASPsiRNA information siRNA Id:aspsirna3075
siRNA Name: AR-mt1-siRNA
Mismatch information Wild siRNA (As strand 3'->5'):CGUACUUGGCCUCCGGGUA
ASP-siRNA (As strand 3'->5'):CGUACUUGACCUCCGGGUAGG
Mismatch position in siRNA: A13G
Gene Information Gene Name Tumor protein p53
Target Sequence (5'->3'):GCATGAACCGGAGGCCCATCCTCACCATCATCA
Wild allele (5'->3'):GCAUGAACCGGAGGCCCAU
Mutant allele (5'->3'):GCAUGAACUGGAGGCCCAUCC
Position of siRNA on target gene: 936-954
Respective Gene/Protein Resources GenBank Accession:NM_000546
Cytogenic location:17p13.1 
Chromosomal coordinates:17:7,668,401-7,687,549 
UniProt ID:P04637
HUGO ID:11998
Reference SNp(RefSNP): rs58778208
Disease/Mutation information Target Mutation: c.944C>T,p.R249W
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_017013.2:g.18333C>T
Pathogenic status of mutation: Pathogenic
Disease:Cancer
Clinical Resources ClinVar ID:141881
KEGG disease ID:H00881
OMIM ID: 191170
COSMIC: TP53
DECIPHER: TP53
GeneTests: TP53
ASP siRNA details Mutant allele (5'->3'): GCAUGAACUGGAGGCCCAUCC
ASP-siRNA (As strand 3'->5'):CGUACUUGACCUCCGGGUAGG
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): GCAUGAACCGGAGGCCCAU
ASP-siRNA (As strand 3'->5'):CGUACUUGACCUCCGGGUAGG
Percentage efficacy of ASP-siRNA for wild allele:60
Relative difference:15
Wild siRNA details Wild allele (5'->3'): GCAUGAACCGGAGGCCCAU
Wild siRNA (As strand 3'->5'):CGUACUUGGCCUCCGGGUA
Percentage efficacy of wild siRNA for wild allele:70
Wild allele (5'->3'): GCAUGAACCGGAGGCCCAU
ASP-siRNA (As strand 3'->5'):CGUACUUGACCUCCGGGUAGG
Percentage efficacy of Wild sirna for mutant allele:60
Relative difference :10
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: H1299
Experimental technique used: Western blot
Transfection method: Lipofectamine
siRNA expression method: MWG Biotech
Post-transfection duration: 24 hours
Concentration used:400 ng
Reference:12403821
Delivery method:shRNA vector mediated delivery
Article title:Synthetic small inhibiting RNAs: Efficient tools to inactivate oncogenic mutations and restore p53 pathways
Authors:Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A
Journal Reference:Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28