aspsirna3083 details

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ASPsiRNA information siRNA Id:aspsirna3083
siRNA Name: R273H-#6
Mismatch information Wild siRNA (As strand 3'->5'):GAAACUCCACGCACAAACACG
ASP-siRNA (As strand 3'->5'):GAAACUCCACGUACAAACACG
Mismatch position in siRNA: U10C
Gene Information Gene Name Tumor protein p53
Target Sequence (5'->3'):CUUUGAGGUGCGTGUUUGUGCCUGUCCU
Wild allele (5'->3'):CUUUGAGGUGCGUGUUUGUGC
Mutant allele (5'->3'):CUUUGAGGUGCAUGUUUGUGC
Position of siRNA on target gene: 1009-1029
Respective Gene/Protein Resources GenBank Accession:NM_000546.5
Cytogenic location:17p13.1 
Chromosomal coordinates:17:7,668,401-7,687,549 
UniProt ID:P04637
HUGO ID:11998
Reference SNp(RefSNP): rs57315468
Disease/Mutation information Target Mutation: NC_000005.10:g.144594367C>A
Matation type/variant: missense variant
Pathogenic status of mutation: Pathogenic
Disease:Cancer
Clinical Resources ClinVar ID:142273
KEGG disease ID:H00881
OMIM ID: 191170
COSMIC: TP53
DECIPHER: TP53
GeneTests: TP53
ASP siRNA details Mutant allele (5'->3'): CUUUGAGGUGCAUGUUUGUGC
ASP-siRNA (As strand 3'->5'):GAAACUCCACGUACAAACACG
Percentage efficacy of ASP-siRNA for mutant allele:65
Wild allele (5'->3'): CUUUGAGGUGCGUGUUUGUGC
ASP-siRNA (As strand 3'->5'):GAAACUCCACGUACAAACACG
Percentage efficacy of ASP-siRNA for wild allele:25
Relative difference:40
Wild siRNA details Wild allele (5'->3'): CUUUGAGGUGCGUGUUUGUGC
Wild siRNA (As strand 3'->5'):GAAACUCCACGCACAAACACG
Percentage efficacy of wild siRNA for wild allele:70
Wild allele (5'->3'): CUUUGAGGUGCGUGUUUGUGC
ASP-siRNA (As strand 3'->5'):GAAACUCCACGUACAAACACG
Percentage efficacy of Wild sirna for mutant allele:50
Relative difference :20
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: MG-R273H
Experimental technique used: Western blot
Transfection method: INTERFERin
siRNA expression method: Integrated DNA Technology
Post-transfection duration: 24 hours
Concentration used:80 nM
Reference:26700961
Delivery method:shRNA-encoding lentiviral vectors
Article title:Allele-specific silencing of mutant p53 attenuates dominant- negative and gain-of-function activities
Authors:Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A
Journal Reference:Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28