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| ASPsiRNA information |
siRNA Id: | aspsirna3083
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|
siRNA Name: | R273H-#6
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GAAACUCCACGCACAAACACG
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|
ASP-siRNA (As strand 3'->5'): | GAAACUCCACGUACAAACACG
|
|
Mismatch position in siRNA: | U10C |
| Gene Information |
Gene Name | Tumor protein p53 |
|
Target Sequence (5'->3'): | CUUUGAGGUGCGTGUUUGUGCCUGUCCU
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|
Wild allele (5'->3'): | CUUUGAGGUGCGUGUUUGUGC
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Mutant allele (5'->3'): | CUUUGAGGUGCAUGUUUGUGC
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|
Position of siRNA on target gene: | 1009-1029
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000546.5 |
| Cytogenic location: | 17p13.1 |
| Chromosomal coordinates: | 17:7,668,401-7,687,549 |
| UniProt ID: | P04637 |
| HUGO ID: | 11998 |
|
Reference SNp(RefSNP): | rs57315468 |
| Disease/Mutation information |
Target Mutation: | NC_000005.10:g.144594367C>A
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Matation type/variant: | missense variant
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Pathogenic status of mutation: | Pathogenic
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 142273 |
|
KEGG disease ID: | H00881 |
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OMIM ID: | 191170 |
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COSMIC: | TP53 |
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DECIPHER: | TP53 |
|
GeneTests: | TP53 |
| ASP siRNA details |
Mutant allele (5'->3'): | CUUUGAGGUGCAUGUUUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | GAAACUCCACGUACAAACACG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 65
|
|
Wild allele (5'->3'): | CUUUGAGGUGCGUGUUUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | GAAACUCCACGUACAAACACG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 25
|
|
Relative difference: | 40
|
| Wild siRNA details |
Wild allele (5'->3'): | CUUUGAGGUGCGUGUUUGUGC
|
|
Wild siRNA (As strand 3'->5'): | GAAACUCCACGCACAAACACG
|
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
|
Wild allele (5'->3'): | CUUUGAGGUGCGUGUUUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | GAAACUCCACGUACAAACACG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 50
|
|
Relative difference : | 20
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | MG-R273H |
| Experimental technique used: | Western blot |
| Transfection method: | INTERFERin |
| siRNA expression method: | Integrated DNA Technology |
| Post-transfection duration: | 24 hours |
| Concentration used: | 80 nM |
| Reference: | 26700961 |
| Delivery method: | shRNA-encoding lentiviral vectors |
'| Article title: | Allele-specific silencing of mutant p53 attenuates dominant- negative and gain-of-function activities |
'| Authors: | Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A |
'| Journal Reference: | Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28 |
'
