| | |
ASPsiRNA information |
siRNA Id: | aspsirna3092
|
siRNA Name: | R273H-#3
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | CUCCACGCACAAACACGGA
|
ASP-siRNA (As strand 3'->5'): | CUCCACGUACAAACACGGA
|
Mismatch position in siRNA: | U12C |
Gene Information |
Gene Name | Tumor protein p53 |
Target Sequence (5'->3'): | CUUUGAGGUGCGTGUUUGUGCCUGUCCU
|
Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
|
Mutant allele (5'->3'): | GAGGUGCAUGUUUGUGCCU
|
Position of siRNA on target gene: | 1013-1031
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000546.5 |
Cytogenic location: | 17p13.1 |
Chromosomal coordinates: | 17:7,668,401-7,687,549 |
UniProt ID: | P04637 |
HUGO ID: | 11998 |
Reference SNp(RefSNP): | rs57315468 |
Disease/Mutation information |
Target Mutation: | NC_000005.10:g.144594367C>A
|
Matation type/variant: | missense variant
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Cancer |
Clinical Resources |
ClinVar ID: | 142273 |
KEGG disease ID: | H00881 |
OMIM ID: | 191170 |
COSMIC: | TP53 |
DECIPHER: | TP53 |
GeneTests: | TP53 |
ASP siRNA details |
Mutant allele (5'->3'): | GAGGUGCAUGUUUGUGCCU
|
ASP-siRNA (As strand 3'->5'): | CUCCACGUACAAACACGGA
|
Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
|
ASP-siRNA (As strand 3'->5'): | CUCCACGUACAAACACGGA
|
Percentage efficacy of ASP-siRNA for wild allele: | 20
|
Relative difference: | 50
|
Wild siRNA details |
Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
|
Wild siRNA (As strand 3'->5'): | CUCCACGCACAAACACGGA
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
Wild allele (5'->3'): | GAGGUGCGUGUUUGUGCCU
|
ASP-siRNA (As strand 3'->5'): | CUCCACGUACAAACACGGA
|
Percentage efficacy of Wild sirna for mutant allele: | 50
|
Relative difference : | 20
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | SW48 |
Experimental technique used: | Western blot |
Transfection method: | INTERFERin |
siRNA expression method: | Integrated DNA Technology |
Post-transfection duration: | 24 hours |
Concentration used: | 80 nM |
Reference: | 26700961 |
Delivery method: | shRNA-encoding lentiviral vectors |
'Article title: | Allele-specific silencing of mutant p53 attenuates dominant- negative and gain-of-function activities |
'Authors: | Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A |
'Journal Reference: | Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28 |
'