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| ASPsiRNA information |
siRNA Id: | aspsirna3104
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|
siRNA Name: | R175H-#2
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGCGA
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ASP-siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGUGA
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|
Mismatch position in siRNA: | U3C |
| Gene Information |
Gene Name | Tumor protein p53 |
|
Target Sequence (5'->3'): | UGACGGAGGUUGUGAGGCGCUGCCCCCACCAUGA
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Wild allele (5'->3'): | UGACGGAGGUUGUGAGGCGCU
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Mutant allele (5'->3'): | UGACGGAGGUUGUGAGGCACU
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|
Position of siRNA on target gene: | 708-728
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000546.5 |
| Cytogenic location: | 17p13.1 |
| Chromosomal coordinates: | 17:7,668,401-7,687,549 |
| UniProt ID: | P04637 |
| HUGO ID: | 11998 |
|
Reference SNp(RefSNP): | rs28934578 |
| Disease/Mutation information |
Target Mutation: | c.524G>A ,p.Arg175His
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Matation type/variant: | missense variant
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Mutation at gene level: | NG_017013.2:g.17463G>A
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Pathogenic status of mutation: | Pathogenic
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 12374 |
|
KEGG disease ID: | H00881 |
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OMIM ID: | 191170 |
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COSMIC: | TP53 |
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DECIPHER: | TP53 |
|
GeneTests: | TP53 |
| ASP siRNA details |
Mutant allele (5'->3'): | UGACGGAGGUUGUGAGGCACU
|
|
ASP-siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGUGA
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 83
|
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Wild allele (5'->3'): | UGACGGAGGUUGUGAGGCGCU
|
|
ASP-siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGUGA
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 15
|
|
Relative difference: | 68
|
| Wild siRNA details |
Wild allele (5'->3'): | UGACGGAGGUUGUGAGGCGCU
|
|
Wild siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGCGA
|
|
Percentage efficacy of wild siRNA for wild allele: | 60
|
|
Wild allele (5'->3'): | UGACGGAGGUUGUGAGGCGCU
|
|
ASP-siRNA (As strand 3'->5'): | ACUGCCUCCAACACUCCGUGA
|
|
Percentage efficacy of Wild sirna for mutant allele: | 45
|
|
Relative difference : | 15
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | CAL33 |
| Experimental technique used: | Western blot |
| Transfection method: | INTERFERin |
| siRNA expression method: | Integrated DNA Technology |
| Post-transfection duration: | 24 hours |
| Concentration used: | 80 nM |
| Reference: | 26700961 |
| Delivery method: | shRNA-encoding lentiviral vectors |
'| Article title: | Allele-specific silencing of mutant p53 attenuates dominant- negative and gain-of-function activities |
'| Authors: | Martinez LA, Naguibneva I, Lehrmann H, Vervisch A, Tchenio T, Lozano G, Harel-Bellan A |
'| Journal Reference: | Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14849-54. Epub 2002 Oct 28 |
'
