aspsirna3113 details

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ASPsiRNA information siRNA Id:aspsirna3113
siRNA Name: K6a siRNA 2
Mismatch information Wild siRNA (As strand 3'->5'):NA
ASP-siRNA (As strand 3'->5'):GAUCAGGAUACUACAUGUU
Gene Information Gene Name Keratin 6a (KRT6a)
Target Sequence (5'->3'):CCCTCAACCTGCAAATCGATCCCACCATCC
Wild allele (5'->3'):NA
Mutant allele (5'->3'):CUAGUCCUAUGAUGUACAA
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_005554.3
Cytogenic location:12q13.13
Chromosomal coordinates:12:52,880,957-52,887,180
UniProt ID:P02538
HUGO ID:6443
Reference SNp(RefSNP): NA
Disease/Mutation information Target Mutation: NA
Matation type/variant: NA
Mutation at gene level: NA
Pathogenic status of mutation: Pathogenic
Disease:Pachyonychia congenita (PC)
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00684
OMIM ID: 167200
COSMIC: KRT6A
DECIPHER: KRT6A
GeneTests: KRT6A
ASP siRNA details Mutant allele (5'->3'): CUAGUCCUAUGAUGUACAA
ASP-siRNA (As strand 3'->5'):GAUCAGGAUACUACAUGUU
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GAUCAGGAUACUACAUGUU
Percentage efficacy of ASP-siRNA for wild allele:30
Relative difference:45
Wild siRNA details Wild allele (5'->3'): NA
Wild siRNA (As strand 3'->5'):NA
Percentage efficacy of wild siRNA for wild allele:50
Wild allele (5'->3'): NA
ASP-siRNA (As strand 3'->5'):GAUCAGGAUACUACAUGUU
Percentage efficacy of Wild sirna for mutant allele:35
Relative difference :15
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: 293FT
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:1 nM
Reference:17762855
Delivery method:shRNA-encoding vector mediated delivery
Article title:Development of Therapeutic siRNAs for Pachyonychia Congenita
Authors:Smith FJ, Hickerson RP, Sayers JM, Reeves RE, Contag CH, Leake D, Kaspar RL, McLean WH
Journal Reference:J Invest Dermatol. 2008 Jan;128(1):50-8. Epub 2007 Aug 30