aspsirna3148 details

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ASPsiRNA information siRNA Id:aspsirna3148
siRNA Name: siRNA-T15
Mismatch information Wild siRNA (As strand 3'->5'):CGACGAAACUCCGGGCCUA
ASP-siRNA (As strand 3'->5'):CGACGAAACUCCGGACCUA
Mismatch position in siRNA: A5G
Gene Information Gene Name tumor protein p63 (TP63)
Target Sequence (5'->3'):TCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCC
Wild allele (5'->3'):GCUGCUUUGAGGCCCGGAU
Mutant allele (5'->3'):GCUGCUUUGAGGCCUGGAU
Position of siRNA on target gene: 872-890
Respective Gene/Protein Resources GenBank Accession:NM_001114980
Cytogenic location:3q28 
Chromosomal coordinates:3:189,566,860-189,897,278 
UniProt ID:Q9H3D4
HUGO ID:15979
Reference SNp(RefSNP): rs12190883
Disease/Mutation information Target Mutation: c.727C>T, p.Arg243Trp
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_007550.1:g.237953C>T
Pathogenic status of mutation: Pathogenic
Disease:Ectrodactily-ectodermal dysplasia and cleft lip/palate (EEC)
Clinical Resources ClinVar ID:6527
KEGG disease ID:NA
OMIM ID: 604292
COSMIC: TP63
DECIPHER: TP63
GeneTests: TP63
ASP siRNA details Mutant allele (5'->3'): GCUGCUUUGAGGCCUGGAU
ASP-siRNA (As strand 3'->5'):CGACGAAACUCCGGACCUA
Percentage efficacy of ASP-siRNA for mutant allele:85
Wild allele (5'->3'): GCUGCUUUGAGGCCCGGAU
ASP-siRNA (As strand 3'->5'):CGACGAAACUCCGGACCUA
Percentage efficacy of ASP-siRNA for wild allele:40
Relative difference:45
Wild siRNA details Wild allele (5'->3'): GCUGCUUUGAGGCCCGGAU
Wild siRNA (As strand 3'->5'):CGACGAAACUCCGGGCCUA
Percentage efficacy of wild siRNA for wild allele:60
Wild allele (5'->3'): GCUGCUUUGAGGCCCGGAU
ASP-siRNA (As strand 3'->5'):CGACGAAACUCCGGACCUA
Percentage efficacy of Wild sirna for mutant allele:70
Relative difference :-10
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK-293E
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Chemically synthesized
Post-transfection duration: 48 hours
Concentration used:40 nM
Reference:27195674
Delivery method:Transfection
Article title:Allele-specific silencing of EEC p63 mutant R304W restores p63 transcriptional activity
Authors:Novelli F, Lena AM, Panatta E, Nasser W, Shalom-Feuerstein R, Candi E, Melino G.
Journal Reference:Cell Death Dis. 2016 May 19;7:e2227. doi: 10.1038/cddis.2016.118