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| ASPsiRNA information |
siRNA Id: | aspsirna3162
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siRNA Name: | miCAG3 (5'->3')
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GUUGCUGCUGCUGCUGCUGCUG
|
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ASP-siRNA (As strand 3'->5'): | GUUGCUGCAAAUGCUGCUGCUG
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|
Mismatch position in siRNA: | A9U,A10G,A11C |
| Gene Information |
Gene Name | Hungtintin (HTT) |
|
Target Sequence (5'->3'): | NA
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|
Wild allele (5'->3'): | CAACGACGACGACGACGACGAC
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Mutant allele (5'->3'): | CAACGACGUUUACGACGACGAC
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Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | P42858 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | (CAG)81
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Matation type/variant: | microsatellite
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Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
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KEGG disease ID: | H00059 |
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OMIM ID: | 143100 |
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COSMIC: | HTT |
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DECIPHER: | HTT |
|
GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | CAACGACGUUUACGACGACGAC
|
|
ASP-siRNA (As strand 3'->5'): | GUUGCUGCAAAUGCUGCUGCUG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 50
|
|
Wild allele (5'->3'): | CAACGACGACGACGACGACGAC
|
|
ASP-siRNA (As strand 3'->5'): | GUUGCUGCAAAUGCUGCUGCUG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 20
|
|
Relative difference: | 30
|
| Wild siRNA details |
Wild allele (5'->3'): | CAACGACGACGACGACGACGAC
|
|
Wild siRNA (As strand 3'->5'): | GUUGCUGCUGCUGCUGCUGCUG
|
|
Percentage efficacy of wild siRNA for wild allele: | 70
|
|
Wild allele (5'->3'): | CAACGACGACGACGACGACGAC
|
|
ASP-siRNA (As strand 3'->5'): | GUUGCUGCAAAUGCUGCUGCUG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 45
|
|
Relative difference : | 25
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK-293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| Concentration used: | 40 nM |
| Reference: | 25849618 |
| Delivery method: | miRNA-expressing plasmids |
'| Article title: | Artificial miRNAs Targeting Mutant Huntingtin Show Preferential Silencing In Vitro and In Vivo |
'| Authors: | Monteys AM,Wilson MJ,Boudreau RL,Spengler RM,Davidson BL |
'| Journal Reference: | Mol Ther Nucleic Acids. 2015 Apr 7;4:e234. doi: 10.1038/mtna.2015.7 |
'
